Document Detail


Anti-transfer RNA antibodies in two patients with pulmonary fibrosis, Raynaud's phenomenon and polyarthritis.
MedLine Citation:
PMID:  9641513     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Patient W.S. (a 61-year-old woman) and patient T.M. (a 41-year-old man) developed recurrent fevers, polyarthritis, Raynaud's phenomenon and interstitial pulmonary fibrosis without apparent polymyositis. From HeLa cell extracts, sera from both patients immunoprecipitated all species of intact and deproteinised tRNAs. To identify the antibody binding site more precisely, tRNAs transcribed in vitro from cloned Escherichia coli tRNA genes and various mutants were prepared and used as antigens for immunoprecipitation. When the TpsiC loop, or the D loop were deleted, such mutants were not bound by both sera, suggesting that the D and TpsiC loops were required for antibody binding. Abrogation of tRNA binding occurred when 18G of tRNATrp was replaced with 18A to break the tertiary L-shape structure of tRNA. These results strongly suggest that sera from W.S. and T.M. recognise the tertiary conformation of L-shaped tRNA which is constructed with both D and TpsiC loops. These autoantibodies may also serve as a marker for a new subset of patients with connective tissue diseases that is distinct from anti-aminoacyl-tRNA synthetase syndrome.
Authors:
Y Ohosone; M Matsumura; J Chiba; S Nagaoka; Y Matsuoka; S Irimajiri; T Mimori
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Publication Detail:
Type:  Case Reports; Journal Article    
Journal Detail:
Title:  Clinical rheumatology     Volume:  17     ISSN:  0770-3198     ISO Abbreviation:  Clin. Rheumatol.     Publication Date:  1998  
Date Detail:
Created Date:  1998-08-11     Completed Date:  1998-08-11     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8211469     Medline TA:  Clin Rheumatol     Country:  BELGIUM    
Other Details:
Languages:  eng     Pagination:  144-7     Citation Subset:  IM    
Affiliation:
Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Arthritis / immunology*
Autoantibodies / blood*,  metabolism
Binding Sites, Antibody
Escherichia coli / genetics
Female
Humans
Male
Middle Aged
Mutation
Precipitin Tests
Pulmonary Fibrosis / immunology*
RNA, Bacterial / metabolism
RNA, Transfer / genetics,  immunology*,  metabolism
Raynaud Disease / immunology*
Chemical
Reg. No./Substance:
0/Autoantibodies; 0/Binding Sites, Antibody; 0/RNA, Bacterial; 9014-25-9/RNA, Transfer

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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