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Anti-quorum sensing activity of Psidium guajava L. flavonoids against Chromobacterium violaceum and Pseudomonas aeruginosa PAO1.
MedLine Citation:
PMID:  24698116     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Psidium guajava L., a traditionally used medicinal plant was explored for anti-quorum sensing (QS) activity. Anti-QS activity of flavonoid fraction (FL) of P. guajava leaves was determined using biosensor bioassay with Chromobacterium violaceum CV026. Detailed investigation on the effect of FL-fraction on QS-regulated violacein production in C. violaceum ATCC12472 and pyocyanin production, proteolytic, elastolytic activities, swarming motility and biofilm formation in Pseudomonas aeruginosa PAO1 were carried out using standard methods. Possible mechanism of QS-inhibition was studied by assessing the violacein production in response to AHL synthesis in presence of FL-fraction in C. violaceum ATCC31532 and by evaluating the induction of violacein in the mutant C. violaceum CV026 by the AHL extracted from the culture supernatants of C. violaceum 31532. Active compounds in the FL-fraction were identified using LC-MS analysis. Inhibition of violacein production by FL-fraction in C. violaceum CV026 biosensor bioassay indicated the possible anti-QS activity. FL-fraction showed concentration-dependent decrease in the violacein production in C. violaceum 12472 and inhibited pyocyanin production, proteolytic, elastolytic activities, swarming motility and biofilm formation in P. aeruginosa PAO1. Interestingly, FL-fraction did not inhibit AHL synthesis as the AHL extracted from the cultures of C. violaceum 31532 grown in presence of FL-fraction could induce violacein in the mutant C. violaceum CV026. LC-MS analysis revealed the presence of quercetin and quercetin-3-O-arabinoside in the FL-fraction. Both quercetin and quercetin-3-O-arabinoside inhibited violacein production in C. violaceum 12472 at 50 µg/ml and 100 µg/ml respectively. Results of this study provide scope for further research to exploit these active molecules as anti-QS agents.
Authors:
Vasavi Halkare Suryanarayana; Arun Ananthapadmanabha Bhagawath; Rekha Punchapady-Devasya
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-4-2
Journal Detail:
Title:  Microbiology and immunology     Volume:  -     ISSN:  1348-0421     ISO Abbreviation:  Microbiol. Immunol.     Publication Date:  2014 Apr 
Date Detail:
Created Date:  2014-4-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703966     Medline TA:  Microbiol Immunol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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This article is protected by copyright. All rights reserved.
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