Document Detail


Anti-proliferative effects of cucurmosin on human hepatoma HepG2 cells.
MedLine Citation:
PMID:  21964700     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We extracted cucurmosin (CUS) from the sarcocarp of Cucrubita moschata (pumpkin). Recently, a number of studies have indicated that CUS has cytotoxic properties and induces apoptosis in a number of human tumor cells. However, the detailed mechanisms are largely unknown. The aim of this study was to confirm CUS's anticancer activity on human hepatoma HepG2 cells in vitro and in vivo, and to elucidate the mechanism of its activity. MTT was used to detect the cytotoxic effects of CUS. Flow cytometry was used to analyze cell apoptosis and the cell cycle. Transmission electron microscopy was used to observe the morphology of apoptotic cells. Western blot analysis was performed to measure the protein expression of bax, bcl-2 and procaspase-3. The established orthotopic transplantation models of human hepatoma in NOD/SCID mice were tested for anticancer activities in vivo. The results showed that CUS inhibited the proliferation of HepG2 cells in vitro and in vivo. CUS induced apoptosis and arrested the cell cycle. In addition, CUS increased the protein expression of bax, but decreased the bcl-2 and procaspase-3 expression in HepG2 cells. Our data indicate that CUS has potential anticancer activity for human hepatoma, which can be attributed in part to its inhibition of proliferation and apoptosis, induced by decreasing the bcl-2:bax ratio and caspase-3 activation.
Authors:
Jieming Xie; Wenzhong Que; Huili Liu; Mei Liu; Aiqin Yang; Minghuang Chen
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-09-30
Journal Detail:
Title:  Molecular medicine reports     Volume:  5     ISSN:  1791-3004     ISO Abbreviation:  Mol Med Rep     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2011-10-26     Completed Date:  2012-02-27     Revised Date:  2013-02-22    
Medline Journal Info:
Nlm Unique ID:  101475259     Medline TA:  Mol Med Rep     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  196-201     Citation Subset:  IM    
Affiliation:
College of Pharmacy, Fujian Medical University, Taijiang, Fuzhou, Fujian 350005, PR China. xiejm2010@163.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Antineoplastic Agents / chemistry,  pharmacology*
Apoptosis / drug effects
Carcinoma, Hepatocellular / mortality,  ultrastructure
Caspase 3 / metabolism
Cell Cycle Checkpoints / drug effects
Cell Proliferation / drug effects
Cell Survival / drug effects
Hep G2 Cells
Humans
Mice
Mice, Inbred NOD
Neoplasm Transplantation
Plant Proteins / chemistry,  pharmacology*
Protein Structure, Tertiary
Proto-Oncogene Proteins c-bcl-2 / metabolism
bcl-2-Associated X Protein / metabolism
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Plant Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein; 0/cucurmosin protein, Cucurbita moschata; EC 3.4.22.-/Caspase 3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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