Document Detail

Anti-proliferative effect of 20-hydroxyecdysone in a lepidopteran cell line.
MedLine Citation:
PMID:  11755067     Owner:  NLM     Status:  MEDLINE    
Ecdysteroids are steroid hormones involved in the epidermal growth of arthropods, controlling cell proliferation and further differentiation of target cells. The epidermal cell line IAL-PID2, established from imaginal discs of the Indian meal moth Plodia interpunctella kept its sensitivity to ecdysteroids in vitro, cells being able to respond to them by cytological and biochemical changes. When added to the culture medium, 20-hydroxyecdysone (20E) stopped cell proliferation and induced formation of epithelial-like aggregates. In order to better understand the cellular sequence of ecdysteroids signalling in epidermal cells we used the IAL-PID2 cell line for in vitro investigations of cytological events induced by the moulting hormone. After a 40 h serum deprivation, formazan assay (XTT) was routinely used to evaluate anti-proliferative effects of 20E during cell cycle. We established a more precise timing of the period of cell sensitivity to the hormone during the cell cycle, by the use of the mitotic index and the BrdU incorporation test. These in vitro assays were performed in parallel with the description of some hormone dependant cytological events, using immunofluorescent labelling with anti-beta tubulin/FITC antibodies and DNA staining.
Stéphanie Auzoux-Bordenave; Philippe-Jacques Hatt; Patrick Porcheron
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Insect biochemistry and molecular biology     Volume:  32     ISSN:  0965-1748     ISO Abbreviation:  Insect Biochem. Mol. Biol.     Publication Date:  2002 Feb 
Date Detail:
Created Date:  2001-12-28     Completed Date:  2002-03-12     Revised Date:  2009-11-03    
Medline Journal Info:
Nlm Unique ID:  9207282     Medline TA:  Insect Biochem Mol Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  217-23     Citation Subset:  IM    
Laboratoire de Physiologie Cellulaire des Invertébrés, Université Paris VI6, 12 rue Cuvier, 75 005 Paris, France.
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MeSH Terms
Cell Division / drug effects*
Cell Line
Ecdysterone / pharmacology*
Epidermis / cytology
Fluorescent Antibody Technique
Moths / cytology
S Phase
Tubulin / metabolism
Reg. No./Substance:
0/Tubulin; 5289-74-7/Ecdysterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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