Document Detail

Anti-proliferative and apoptosis-inducible activity of Sarcodonin G from Sarcodon scabrosus in HeLa cells.
MedLine Citation:
PMID:  19082491     Owner:  NLM     Status:  MEDLINE    
There is an ongoing search for plant-derived diterpenes, especially for diterpenes with anti-inflammatory activity that also have anti-proliferative effects on human cancer cells. A cyathane-type diterpene, Sarcodonin G (SG), isolated from the mushroom Sarcodon scabrosus and already reported to have anti-inflammatory activity, inhibited proliferation of HeLa cells to the greatest extent among 4 cyathane diterpenes tested. SG showed an IC50 (50% inhibition concentration) of 20 microM, estimated by MTT assay 2 days after culture of cells with the chemical. SG treatment of HeLa cells resulted in dose-dependent generation of apoptotic events such as DNA-laddering (< or =100 microM). Moreover, SG-treated HeLa cells showed activation of caspase-3 and caspase-9 and increase of Bax/Bcl-2 ratios, as analyzed by Western blot analysis. The anti-proliferative effects of SG treatment on HeLa cells were lessened by a caspase inhibitor, Z-VAD-FMK. SG also showed anti-proliferative effects toward 5 other human cancer cell lines with IC50 values of 20-40 microM. Because of these anti-proliferative effects via possible caspase activation, SG holds promise of being a novel anti-proliferative agent deserving further investigation.
Mei Dong; Shi-Ping Chen; Kazuko Kita; Yoshinobu Ichimura; Wen-Zhi Guo; Sa Lu; Shigeru Sugaya; Takaki Hiwasa; Masaki Takiguchi; Naomi Mori; Atsushi Kashima; Keiji Morimura; Mitsuru Hirota; Nobuo Suzuki
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of oncology     Volume:  34     ISSN:  1019-6439     ISO Abbreviation:  Int. J. Oncol.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-12-16     Completed Date:  2009-02-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9306042     Medline TA:  Int J Oncol     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  201-7     Citation Subset:  IM    
Department of Medicinal Natural Product Chemistry, School of Pharmaceutical Sciences, Hebei Medical University, Shijiazhuang 050017, P.R. China.
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MeSH Terms
Apoptosis / drug effects*
Caspase 3 / metabolism
Caspase 9 / metabolism
Cell Cycle / drug effects
Cell Proliferation / drug effects*
Diterpenes / pharmacology*
Enzyme Activation / drug effects
Enzyme Inhibitors / pharmacology
Hela Cells
Neoplasms / drug therapy,  metabolism,  pathology
Proto-Oncogene Proteins c-bcl-2 / metabolism
bcl-2-Associated X Protein / metabolism
Reg. No./Substance:
0/Diterpenes; 0/Enzyme Inhibitors; 0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein; 0/sarcodonin G; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 9

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