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Anti-miR-155 oligonucleotide enhances chemosensitivity of U251 cell to taxol by inducing apoptosis.
MedLine Citation:
PMID:  22276743     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Substantial data indicate that the oncogene microRNA-155 is significantly elevated in glioblastoma multiforme (GBM) and regulates multiple genes associated with cancer cell proliferation, apoptosis, and invasiveness. Thus, miR-155 can theoretically become a target to enhance the chemotherapeutic effect in cancer therapy. So far, the effect of down-regulating miR-155 to enhance the chemotherapeutic effect to taxol has not been studied in human GBM. Human GBM U251 cell were treated with taxol and the miR-155 inhibitor, alone or in combination. The 50% inhibitory concentration and cell viability were determined by the MTT assay. Annexin V/PI staining was performed, and apoptosis and the cell cycle were evaluated by flow cytometry analysis. Expression of miR-155 was real-time PCR and western blotting was performed to evaluate malignancy related protein alteration. IC50 values were dramatically decreased in cells treated with miR-155 inhibitor combine with taxol, to a greater extent than those treated with taxol alone. Furthermore, the miR-155 inhibitor significantly enhaced apoptosis in U251 cell. Interestingly, the above data suggested that in GBM cells, miR-155 blockage increased the chemosensitivity to taxol. The data strongly suggested that a regulatory loop between miR-155 might provide an insight into the mechanism of modulating EAG1 signaling. Taken together, the miR-155 inhibitor could enhance the chemosensitivity of human glioblastoma cells to taxol. A combination of miR-155 inhibitor and taxol could be an effective therapeutic strategy for controlling the growth of GBM by inhibiting EAG1 expression.
Authors:
Wei Meng; Ling Jiang; Lin Lu; Haiyan Hu; Hailang Yu; Dapeng Ding; Kun Xiao; Wenling Zheng; Hongbo Guo; Wenli Ma
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-26
Journal Detail:
Title:  Cell biology international     Volume:  -     ISSN:  1095-8355     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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