| Anti-miR-155 oligonucleotide enhances chemosensitivity of U251 cell to taxol by inducing apoptosis. | |
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MedLine Citation:
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PMID: 22276743 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Substantial data indicate that the oncogene microRNA-155 is significantly elevated in glioblastoma multiforme (GBM) and regulates multiple genes associated with cancer cell proliferation, apoptosis, and invasiveness. Thus, miR-155 can theoretically become a target to enhance the chemotherapeutic effect in cancer therapy. So far, the effect of down-regulating miR-155 to enhance the chemotherapeutic effect to taxol has not been studied in human GBM. Human GBM U251 cell were treated with taxol and the miR-155 inhibitor, alone or in combination. The 50% inhibitory concentration and cell viability were determined by the MTT assay. Annexin V/PI staining was performed, and apoptosis and the cell cycle were evaluated by flow cytometry analysis. Expression of miR-155 was real-time PCR and western blotting was performed to evaluate malignancy related protein alteration. IC50 values were dramatically decreased in cells treated with miR-155 inhibitor combine with taxol, to a greater extent than those treated with taxol alone. Furthermore, the miR-155 inhibitor significantly enhaced apoptosis in U251 cell. Interestingly, the above data suggested that in GBM cells, miR-155 blockage increased the chemosensitivity to taxol. The data strongly suggested that a regulatory loop between miR-155 might provide an insight into the mechanism of modulating EAG1 signaling. Taken together, the miR-155 inhibitor could enhance the chemosensitivity of human glioblastoma cells to taxol. A combination of miR-155 inhibitor and taxol could be an effective therapeutic strategy for controlling the growth of GBM by inhibiting EAG1 expression. |
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Authors:
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Wei Meng; Ling Jiang; Lin Lu; Haiyan Hu; Hailang Yu; Dapeng Ding; Kun Xiao; Wenling Zheng; Hongbo Guo; Wenli Ma |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-26 |
Journal Detail:
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Title: Cell biology international Volume: - ISSN: 1095-8355 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-26 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9307129 Medline TA: Cell Biol Int Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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