Document Detail

Anti-listerial activity of a polymeric film coated with hybrid coatings doped with Enterocin 416K1 for use as bioactive food packaging.
MedLine Citation:
PMID:  18262299     Owner:  NLM     Status:  MEDLINE    
In this study, Enterocin 416K1, a bacteriocin produced by Enterococcus casseliflavus IM 416K1, was entrapped in an organic-inorganic hybrid coating applied to a LDPE (low-density polyethylene) film for its potential use in the active food packaging field. The antibacterial activity of the coated film was evaluated against Listeria monocytogenes NCTC 10888 by qualitative modified agar diffusion assay, quantitative determination in listeria saline solution suspension and direct contact with artificially contaminated food samples (frankfurters and fresh cheeses) stored at room and refrigeration temperatures. All investigations demonstrated that enterocin-activated coatings have a good anti-listeria activity. Qualitative tests showed a clear zone of inhibition in the indicator lawn in contact with and around the coated film. During the quantitative antibacterial evaluation the L. monocytogenes viable counts decreased to 1.5 log units compared to the control. The inhibitory capability was confirmed also in food-contact assays. In all food samples packed with coated films we observed a significant decrease in L. monocytogenes viable counts in the first 24 h compared to the control. This difference was generally maintained up to the seventh day and then decreased, with the exception of the cheese samples stored at refrigeration temperature.
Ramona Iseppi; Francesco Pilati; Michele Marini; Maurizio Toselli; Simona de Niederhäusern; Elisa Guerrieri; Patrizia Messi; Carla Sabia; Giuliano Manicardi; Immacolata Anacarso; Moreno Bondi
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Publication Detail:
Type:  Journal Article     Date:  2007-12-27
Journal Detail:
Title:  International journal of food microbiology     Volume:  123     ISSN:  0168-1605     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-04-29     Completed Date:  2008-08-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  281-7     Citation Subset:  IM    
Department of Biomedical Sciences, University of Modena and Reggio Emilia, Via Campi 287, 41100 Modena, Italy.
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MeSH Terms
Anti-Bacterial Agents / pharmacology*
Bridged Compounds / pharmacology
Cheese / microbiology*
Colony Count, Microbial
Consumer Product Safety
Enterococcus / metabolism*
Food Contamination / prevention & control
Food Microbiology
Food Packaging / methods*
Food Preservation / methods
Listeria monocytogenes / drug effects*,  growth & development
Time Factors
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Bridged Compounds; 59678-46-5/enterocin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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