Document Detail


Anti-inflammatory effects of orally ingested lactoferrin and glycine in different zymosan-induced inflammation models: evidence for synergistic activity.
MedLine Citation:
PMID:  17996689     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
There is a growing awareness of the interaction of food constituents with the immune system. The present study aims to evaluate the anti-inflammatory effects of two of these nutritional components (glycine and bovine-lactoferrin (b-LF)) using two different mouse models. In a zymosan-induced ear-skin inflammation model both components decreased the inflammatory response locally (ear swelling and inflammatory cytokine concentration in the ears) and systemically (number of TNF-alpha producing spleen cells). Glycine effects (20, 50 or 100 mg/mouse/day) were concentration dependent. B-LF (0.1 or 1 mg/mouse/day) inhibited the inflammatory response although higher doses (5 and 25 mg/mouse/day) were not effective. A combination of b-LF 0.1 mg/mouse/day and glycine 20 or 50 mg/mouse/day counteracted the zymosan-induced ear swelling synergistically and enhanced the decrease in the number of TNF-alpha producing spleen cells of the individual components. In a zymosan-induced acute arthritis model glycine (50 mg/mouse/day) inhibited joint swelling, inflammatory cell infiltration and cartilage proteoglycan depletion. A b-LF dose of 5 mg/mouse/day reduced the zymosan-induced joint swelling without modulating inflammatory cell infiltration and cartilage proteoglycan depletion. The present study indicates that the anti-inflammatory effects of glycine are independent of the used models. B-LF displays a reversed concentration dependency and the activity is model dependent. A combination of glycine and lactoferrin demonstrated a synergistic anti-inflammatory effect on zymosan-induced skin inflammation and an enhanced decrease in the number of TNF-alpha producing spleen cells compared to the effect of the single components. Therefore, this nutritional concept might be a new option for the treatment of chronic inflammatory diseases.
Authors:
Anita Hartog; Inge Leenders; Peter M van der Kraan; Johan Garssen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-10-08
Journal Detail:
Title:  International immunopharmacology     Volume:  7     ISSN:  1567-5769     ISO Abbreviation:  Int. Immunopharmacol.     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-11-12     Completed Date:  2008-01-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100965259     Medline TA:  Int Immunopharmacol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1784-92     Citation Subset:  IM    
Affiliation:
Numico Research, Wageningen, The Netherlands. anita.hartog@numico-research.nl
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MeSH Terms
Descriptor/Qualifier:
Administration, Oral
Animals
Anti-Inflammatory Agents / pharmacology*
Disease Models, Animal
Drug Synergism
Glycine / administration & dosage,  pharmacology*
Inflammation / chemically induced,  drug therapy*
Lactoferrin / administration & dosage,  pharmacology*
Male
Mice
Mice, Inbred BALB C
Proteoglycans / metabolism
Tumor Necrosis Factor-alpha / biosynthesis
Zymosan
Chemical
Reg. No./Substance:
0/Anti-Inflammatory Agents; 0/Lactoferrin; 0/Proteoglycans; 0/Tumor Necrosis Factor-alpha; 56-40-6/Glycine; 9010-72-4/Zymosan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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