| Anti-inflammatory effects of dietary phenolic compounds in an in vitro model of inflamed human intestinal epithelium. | |
| | |
MedLine Citation:
|
PMID: 20816778 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Phenolic compounds (PCs) are considered to possess anti-inflammatory properties and therefore were proposed as an alternative natural approach to prevent or treat chronic inflammatory diseases. However their effects are not fully understood, particularly at the intestinal level. To further understand their mode of action at the molecular level during intestinal inflammation, an in vitro model of inflamed human intestinal epithelium was established. Different representative dietary PCs, i.e. resveratrol, ellagic and ferulic acids, curcumin, quercetin, chrysin, (-)-epigallocatechin-3-gallate (EGCG) and genistein, were selected. To mimic intestinal inflammation, differentiated Caco-2 cells cultivated in bicameral inserts, in a serum-free medium, were treated with a cocktail of pro-inflammatory substances: interleukin (IL)-1β, tumor necrosis factor-α, interferon-γ and lipopolysaccharides. The inflammatory state was characterized by a leaky epithelial barrier (attenuation of the transepithelial electrical resistance) and by an over-expression at the mRNA and protein levels for pro-inflammatory markers, i.e. IL-6, IL-8 and monocyte chemoattractant protein-1 (MCP-1), quantified by ELISA and by gene expression analysis using a low-density array allowing the evaluation of expression level for 46 genes relevant of the intestinal inflammation and functional metabolism. Treatment with PCs, used at a realistic intestinal concentration, did not affect cell permeability. In inflamed cells, the incubation with genistein reduced the IL-6 and MCP-1 overproduction, to ca. 50% of the control, whereas EGCG provoked a decrease in the IL-6 and IL-8 over-secretion, by 50 and 60%, respectively. This occurred for both flavonoids without any concomitant inhibition of the corresponding mRNA expression. All the PCs generated a specific gene expression profile, with genistein the most efficient in the downregulation of the expression, or over-expression, of inflammatory genes notably those linked to the arachidonic metabolism pathway. In conclusion, this study provides evidence that genistein and EGCG downregulate the inflammatory response in inflamed intestinal epithelial cells by a pathway implicating largely a post-transcriptional regulatory mechanism. |
| | |
Authors:
|
Thérèse Sergent; Neil Piront; Julie Meurice; Olivier Toussaint; Yves-Jacques Schneider |
Related Documents
:
|
10807888 - Localisation of cyclooxygenase 1 and cyclooxygenase 2 in helicobacter pylori related ga... 11425208 - Analysis of proteoglycan gene messages in human nasal mucosa and nasal polyp using dot ... 20026908 - The p300/cbp associated factor: is frequently downregulated in intestinal-type gastric ... 12527888 - Altered expression of the ron receptor tyrosine kinase in primary human colorectal aden... 22394678 - Imprinting control region (icr) of the peg3 domain. 16331278 - Lmo4 can interact with smad proteins and modulate transforming growth factor-beta signa... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Chemico-biological interactions Volume: 188 ISSN: 1872-7786 ISO Abbreviation: Chem. Biol. Interact. Publication Date: 2010 Dec |
Date Detail:
|
Created Date: 2010-11-12 Completed Date: 2010-12-21 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0227276 Medline TA: Chem Biol Interact Country: Ireland |
Other Details:
|
Languages: eng Pagination: 659-67 Citation Subset: IM |
Copyright Information:
|
Copyright © 2010 Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
|
Institut des Sciences de la Vie, Louvain-la-Neuve, Belgium. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Anti-Inflammatory Agents
/
pharmacology*,
therapeutic use Caco-2 Cells Diet* Gene Expression Profiling Humans Inflammation / drug therapy, metabolism, pathology Intestinal Mucosa / drug effects*, metabolism, pathology Phenols / pharmacology*, therapeutic use |
| Chemical | |
Reg. No./Substance:
|
0/Anti-Inflammatory Agents; 0/Phenols |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Carnosic acid: a potent chemopreventive agent against oral carcinogenesis.
Next Document: Casearin X exhibits cytotoxic effects in leukemia cells triggered by apoptosis.