| Anti-inflammatory effect of quercetin-loaded microemulsion in the airways allergic inflammatory model in mice. | |
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MedLine Citation:
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PMID: 19892018 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Quercetin is a plant-derived flavonoid widely known by its anti-oxidant and anti-inflammatory properties, but its oral bioavailability is very poor and this becomes difficult to assess its therapeutic potential. Here we have compared the anti-inflammatory effect of quercetin-loaded microemulsion (QU-ME) and quercetin suspension (QU-SP) in an experimental model of airways allergic inflammation. Mice received daily oral doses of QU-ME (3 or 10mg/kg; in an oil-in-water microemulsion content 0.02:0.2:1 of lecithin:castor oil:Solutol HS15((R))), QU-SP [10mg/kg, in carboxymethylcellulose (CMC) 0.5% in water] or vehicle from the 18th to the 22nd day after the first immunization with ovalbumin (OVA). Dexamethasone was used as positive control drug. Every parameter was evaluated in the 22nd day (24h after the second OVA-challenge). We have also tried to assess by HPLC-MS a quercetin metabolite in the blood of rats treated with QU-SP or QU-ME. QU-ME was better orally absorbed when compared with QU-SP. Furthermore, oral administration of QU-SP failed to interfere with leukocyte recruitment, while QU-ME inhibited in a dose-dependent way, the eosinophil recruitment to the bronchoalveolar lavage fluid (BALF). QU-ME also significantly reduced both IL-5 and IL-4 levels, but failed to interfere with CCL11, IFN-gamma and LTB(4) levels. In addition, QU-ME oral treatment inhibited the nuclear transcription factor kappa B (NF-kappaB) activation, P-selectin expression and the mucus production in the lung. The present results show that QU-ME exhibits pronounced anti-inflammatory properties in a murine model of airways allergic inflammation and suggest that it might present therapeutic potential for the airways inflammatory diseases management. |
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Authors:
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Alexandre P Rogerio; Cristiana L Dora; Edin?ia L Andrade; Juliana S Chaves; Luis F C Silva; Elenara Lemos-Senna; Jo?o B Calixto |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-11-03 |
Journal Detail:
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Title: Pharmacological research : the official journal of the Italian Pharmacological Society Volume: 61 ISSN: 1096-1186 ISO Abbreviation: Pharmacol. Res. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-15 Completed Date: 2010-06-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8907422 Medline TA: Pharmacol Res Country: England |
Other Details:
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Languages: eng Pagination: 288-97 Citation Subset: IM |
Copyright Information:
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Copyright 2009 Elsevier Ltd. All rights reserved. |
Affiliation:
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Departamento de Farmacologia, Universidade Federal de Santa Catarina, Campus Universit?rio Trindade, Centro de Ci?ncias Biol?gicas, Florian?polis, SC, Brazil. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Administration, Oral Animals Anti-Inflammatory Agents / administration & dosage*, chemistry, pharmacology* Asthma / blood, chemically induced, drug therapy*, immunology, metabolism Disease Models, Animal Drug Carriers / administration & dosage, chemistry Emulsions / administration & dosage, chemistry Female Inflammation Mediators / metabolism Leukocyte Count Lung / drug effects, immunology, metabolism Mice Mice, Inbred BALB C NF-kappa B / metabolism P-Selectin / metabolism Particle Size Quercetin / administration & dosage*, chemistry, pharmacology* Respiratory Mucosa / drug effects, immunology, metabolism Suspensions |
| Chemical | |
Reg. No./Substance:
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0/Anti-Inflammatory Agents; 0/Drug Carriers; 0/Emulsions; 0/Inflammation Mediators; 0/NF-kappa B; 0/P-Selectin; 0/Suspensions; 117-39-5/Quercetin |
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