| Anti-inflammatory effect of 2-methoxy-4-vinylphenol via the suppression of NF-κB and MAPK activation, and acetylation of histone H3. | |
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MedLine Citation:
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PMID: 22210037 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Although inflammation acts as host defense mechanism against infection or injury and is primarily a self limiting process, inadequate resolution of inflammatory responses leads to various chronic disorders. This work aimed to elucidate the anti-inflammatory effects of 2-methoxy-4-vinylphenol (2M4VP) isolated from pine needles in LPS-stimulated RAW264.7 cells. Some key pro-inflammatory mediators including nitric oxide (NO), prostaglandins (PGE(2)), inducible NO synthase (iNOS), and cyclooxygenase-2 (COX-2) were studied by sandwich ELISA and western blot. In addition, suppression of NF-κB and MAPK activation, and histone acetylation was studied by western blot analysis and immunostaining. 2M4VP dosedependently inhibited NO and PGE(2) production and also blocked LPS-induced iNOS and COX-2 expression. In addition, 2M4VP potently inhibited the translocation of NF-κB p65 into the nucleus by IκB degradation following IκB-α phosphorylation and the phosphorylation of MAPKs such as p38, ERK1/2, and JNK. Also, 2M4VP inhibited hyper-acetylation of histone H3 (Lys9/Lys14) induced by LPS. Taken together, our results suggest that 2M4VP, a naturally occurring phenolic compound, exert potent anti-inflammatory effects by inhibiting LPS-induced NO, PGE(2), iNOS, and COX-2 in RAW264.7 cells. These effects are mediated by suppression of NF-κB and MAPK activation and histone acetylation. |
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Authors:
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Jin Boo Jeong; Se Chul Hong; Hyung Jin Jeong; Jin Suk Koo |
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Publication Detail:
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Type: Journal Article Date: 2011-12-31 |
Journal Detail:
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Title: Archives of pharmacal research Volume: 34 ISSN: 0253-6269 ISO Abbreviation: Arch. Pharm. Res. Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2012-01-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8000036 Medline TA: Arch Pharm Res Country: Korea (South) |
Other Details:
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Languages: eng Pagination: 2109-16 Citation Subset: IM |
Affiliation:
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Medicinal Plant Resources Major, Andong National University, Andong, 760749, Korea, jjb8376@gmail.com. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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