| Anti-inflammatory, antioxidative and matrix metalloproteinase inhibitory properties of 20(R)-ginsenoside Rh2 in cultured macrophages and keratinocytes. | |
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MedLine Citation:
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PMID: 23278699 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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OBJECTIVES: This work aimed to assess the matrix metalloproteinase inhibitory and related pharmacological actions of 20(R)-ginsenoside Rh2 (20(R)-Rh2) in cultured macrophages and keratinocytes. METHODS: In-vitro anti-inflammatory activity of 20(R)-Rh2 was evaluated by analysing nitric oxide (NO) and prostaglandin E2 (PGE2) contents in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. Its antioxidant activity was determined by measuring the level of reactive oxygen species (ROS) in the macrophage and keratinocyte cells. Matrix metalloproteinase-9 (MMP-9) and -2 (MMP-2) activity in the culture medium was detected using zymography. KEY FINDINGS: 20(R)-Rh2 was able to suppress NO, PGE2, ROS and pro-matrix metalloproteinase-9 (pro-MMP-9) levels that were enhanced in the LPS-stimulated murine RAW264.7 macrophage cells. 20(R)-Rh2 also exhibited inhibitory effects on the level of ROS and the activity of MMP-9 and -2 in human HaCat keratinocyte cells without stimulant exposure. 20(R)-Rh2 could suppress the gelatinolytic activity of MMP-9 enhanced by tumour necrosis factor-α in the keratinocytes. CONCLUSIONS: 20(R)-Rh2, a minor stereoisomer of ginsenoside Rh2, possesses matrix metalloproteinase inhibitory, anti-inflammatory and antioxidative activity. |
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Authors:
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Woo-Yong Choi; Hye-Won Lim; Chang-Jin Lim |
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Publication Detail:
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Type: Journal Article Date: 2012-10-22 |
Journal Detail:
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Title: The Journal of pharmacy and pharmacology Volume: 65 ISSN: 2042-7158 ISO Abbreviation: J. Pharm. Pharmacol. Publication Date: 2013 Feb |
Date Detail:
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Created Date: 2013-01-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376363 Medline TA: J Pharm Pharmacol Country: England |
Other Details:
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Languages: eng Pagination: 310-6 Citation Subset: IM |
Copyright Information:
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© 2012 The Authors. JPP © 2012. Royal Pharmaceutical Society. |
Affiliation:
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Department of Biochemistry, College of Natural Sciences, Kangwon National University, Chuncheon, Korea. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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