Document Detail

Anti-inflammatory and antioxidant activity of a medicinal tincture from Pedilanthus tithymaloides.
MedLine Citation:
PMID:  16223515     Owner:  NLM     Status:  MEDLINE    
Pedilanthus tithymaloides (L.) Poit. (Euphorbiaceae) is a low tropical American shrub with a reported wide range of healing properties such as emetic, anti-inflammatory, antibiotic, antiseptic, antihemorrhagic, antiviral, antitumoral, and abortive. In the present study, a tincture from P. tithymaloides collected in Cuba was evaluated for its in vivo anti-inflammatory activity, using the rat paw oedema assay, and for its in vitro scavenging effects on reactive oxygen species (ROS) (HO*, O2*-, HOCl, ROO* and H2O2), reactive nitrogen species (RNS) (ONOO- and *NO), and DPPH* radical. The protein, free amino acid, and phenolic contents of the tincture were also determined. Pertaining to the anti-inflammatory activity, the intraperitoneal administration of the tincture inhibited carrageenan-induced rat paw oedema, whereas in the scavenging assays the tincture showed to be effective against all the assayed ROS and RNS, specially for HO* (IC50 = 345+/-77 microg/mL), O2*- (IC50 = 143+/-7 microg/mL), HOCl (IC50 = 113+/-20 microg/mL), ONOO- (IC50 = 44+/-3 microg/mL), and *NO (IC50 = 54+/-4 microg/mL), but displayed weak activity in the DPPH* assay. The protein content of the tincture was 0.70%, and twenty free amino acids were identified and quantified. The content of total phenolics was 17.4+/-0.15 mg of gallic acid equivalents (GAE)/g dry material. These results provide scientific support for the empirical use of P. tithymaloides tincture as an anti-inflammatory medicine.
Pedro Abreu; Susan Matthew; Tania González; David Costa; Marcela A Segundo; Eduarda Fernandes
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-10-11
Journal Detail:
Title:  Life sciences     Volume:  78     ISSN:  0024-3205     ISO Abbreviation:  Life Sci.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-02-21     Completed Date:  2006-04-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  1578-85     Citation Subset:  IM    
REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
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MeSH Terms
Anti-Inflammatory Agents / therapeutic use*
Antioxidants / therapeutic use*
Biological Assay
Edema / chemically induced,  drug therapy*
Euphorbiaceae / chemistry*
Inhibitory Concentration 50
Plant Extracts / therapeutic use
Rats, Sprague-Dawley
Reactive Oxygen Species / antagonists & inhibitors*
Reg. No./Substance:
0/Anti-Inflammatory Agents; 0/Antioxidants; 0/Plant Extracts; 0/Reactive Oxygen Species

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