| Anti-inflammatory Effects of the Zingiber officinale roscoe Constituent 12-Dehydrogingerdione in Lipopolysaccharide-stimulated Raw 264.7 Cells. | |
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MedLine Citation:
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PMID: 23027684 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Ginger has long been used worldwide as a spice, seasoning, and wine and is also used as a traditional medicine. There have been no previous studies of the potential beneficial effects of the ginger constituent 12-dehydrogingerdione (12-DHGD). We investigated the anti-inflammatory effect of 12-DHGD on lipopolysaccharide (LPS)-stimulated Raw 264.7 cells. The cytotoxicity of 12-DHGD was measured using the MTT assay, and production of prostaglandin E(2) (PGE(2) ) and the inflammatory cytokines interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α was measured by ELISA. Production of nitric oxide (NO) was measured using Griess reagent and expression of cyclooxygenase-2 (COX-2) and inducible NO (iNOS) enzymes was assessed by reverse transcriptase-polymerase chain reaction. Treatment of Raw 264.7 cells with 12-DHGD significantly inhibited LPS-stimulated production of NO (at 12-DHGD concentrations of 150 and 200 ng/ml), IL-6 (at 50, 100, 150, and 200 ng/ml), and PGE(2) (at 200 ng/ml). Consistent with the effects on NO and PGE(2) production, 12-DHGD treatment also inhibited the LPS-stimulated increase in iNOS and COX-2 mRNA levels. However, 12-DHGD did not affect production of IL-1β or TNF-α in response to LPS. 12-DHGD, a constituent of ginger, is a potent inhibitor of proinflammatory mediator production in Raw 264.7 macrophage cells. Copyright © 2012 John Wiley & Sons, Ltd. |
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Authors:
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Young Ah Han; Chang Woo Song; Woo Suk Koh; Gyu Hwan Yon; Young Sup Kim; Shi Yong Ryu; Hoon Jeong Kwon; Kyu Hong Lee |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-10-2 |
Journal Detail:
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Title: Phytotherapy research : PTR Volume: - ISSN: 1099-1573 ISO Abbreviation: Phytother Res Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-10-2 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8904486 Medline TA: Phytother Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 John Wiley & Sons, Ltd. |
Affiliation:
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Inhalation Toxicology Center, Jeonbuk Department of Non-human Primate, Korea Institute of Toxicology, Jeonbuk, 580-185, Korea; College of Human Ecology, Seoul National University, Seoul, 151-742, Korea. |
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