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Anti-inflammatory Effects of the Zingiber officinale roscoe Constituent 12-Dehydrogingerdione in Lipopolysaccharide-stimulated Raw 264.7 Cells.
MedLine Citation:
PMID:  23027684     Owner:  NLM     Status:  Publisher    
Ginger has long been used worldwide as a spice, seasoning, and wine and is also used as a traditional medicine. There have been no previous studies of the potential beneficial effects of the ginger constituent 12-dehydrogingerdione (12-DHGD). We investigated the anti-inflammatory effect of 12-DHGD on lipopolysaccharide (LPS)-stimulated Raw 264.7 cells. The cytotoxicity of 12-DHGD was measured using the MTT assay, and production of prostaglandin E(2) (PGE(2) ) and the inflammatory cytokines interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α was measured by ELISA. Production of nitric oxide (NO) was measured using Griess reagent and expression of cyclooxygenase-2 (COX-2) and inducible NO (iNOS) enzymes was assessed by reverse transcriptase-polymerase chain reaction. Treatment of Raw 264.7 cells with 12-DHGD significantly inhibited LPS-stimulated production of NO (at 12-DHGD concentrations of 150 and 200 ng/ml), IL-6 (at 50, 100, 150, and 200 ng/ml), and PGE(2) (at 200 ng/ml). Consistent with the effects on NO and PGE(2) production, 12-DHGD treatment also inhibited the LPS-stimulated increase in iNOS and COX-2 mRNA levels. However, 12-DHGD did not affect production of IL-1β or TNF-α in response to LPS. 12-DHGD, a constituent of ginger, is a potent inhibitor of proinflammatory mediator production in Raw 264.7 macrophage cells. Copyright © 2012 John Wiley & Sons, Ltd.
Young Ah Han; Chang Woo Song; Woo Suk Koh; Gyu Hwan Yon; Young Sup Kim; Shi Yong Ryu; Hoon Jeong Kwon; Kyu Hong Lee
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-2
Journal Detail:
Title:  Phytotherapy research : PTR     Volume:  -     ISSN:  1099-1573     ISO Abbreviation:  Phytother Res     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8904486     Medline TA:  Phytother Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 John Wiley & Sons, Ltd.
Inhalation Toxicology Center, Jeonbuk Department of Non-human Primate, Korea Institute of Toxicology, Jeonbuk, 580-185, Korea; College of Human Ecology, Seoul National University, Seoul, 151-742, Korea.
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