| Anti-inflammatory effects of alpha linolenic acid on human corneal epithelial cells. | |
| | |
MedLine Citation:
|
PMID: 22669722 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
PURPOSE: Systemic polyunsaturated fatty acids (PUFAs) were shown to improve the symptoms of dry eye syndrome due to their anti-inflammatory effects. This study evaluated the in vitro anti-inflammatory effects of PUFAs on human corneal epithelial (HCE) cells. METHODS: HCE cells were incubated for 2 hours with different concentrations of PUFAs: alpha-linolenic acid (ALA), gamma-linolenic acid (GLA), and linoleic acid (LA). Oleic acid (OA) and dexamethasone (DM) served as negative and positive controls, respectively. Cells were stimulated with either polyinosinic:polycytidylic acid (poly I:C) or lipopolysaccharide (LPS) complex. The protein contents and mRNA expression levels of IL-6, IL-8, IL-1β, and TNF-α were evaluated with multiplex fluorescent bead immunoassay and real-time PCR, respectively. The expression of inhibitory factor-κBα (I-κBα) was evaluated with real-time PCR. RESULTS: The protein and mRNA levels of IL-6, IL-8, IL-1β, and TNF-α were significantly increased after stimulation with LPS or poly I:C. Following treatment with ALA, a significant decrease was demonstrated in the protein content of TNF-α to 23.81% (P < 0.001), IL-6 to 46.71% (P < 0.001), IL-1β to 20.86% (P < 0.05), and IL-8 to 52.21% (P < 0.001). Similar results were demonstrated at the mRNA level. The anti-inflammatory effects of ALA were similar to those of DM for all of the pro-inflammatory cytokines. The ALA inhibition of the pro-inflammatory cytokines was associated with a significant reduction of I-κBα. CONCLUSIONS: ALA may serve as a potent anti-inflammatory agent in ocular surface inflammation. The anti-inflammatory effects of ALA are comparable to those of corticosteroids, and are mediated through NF-κB signal transduction. |
| | |
Authors:
|
Nir Erdinest; Or Shmueli; Yoni Grossman; Haim Ovadia; Abraham Solomon |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-07-03 |
Journal Detail:
|
Title: Investigative ophthalmology & visual science Volume: 53 ISSN: 1552-5783 ISO Abbreviation: Invest. Ophthalmol. Vis. Sci. Publication Date: 2012 Jul |
Date Detail:
|
Created Date: 2012-07-04 Completed Date: 2012-10-16 Revised Date: 2013-01-09 |
Medline Journal Info:
|
Nlm Unique ID: 7703701 Medline TA: Invest Ophthalmol Vis Sci Country: United States |
Other Details:
|
Languages: eng Pagination: 4396-406 Citation Subset: IM |
Affiliation:
|
Department of Ophthalmology, The Agnes Ginges Center for Human Neurogenetics, Hadassah-Hebrew University Medical Center, Jerusalem, Israel. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Anti-Inflammatory Agents, Non-Steroidal
/
pharmacology* Blotting, Western Cell Survival Cells, Cultured Dexamethasone / pharmacology Epithelium, Corneal / drug effects*, metabolism Fluorescent Antibody Technique Humans I-kappa B Proteins / genetics Interleukin-1beta / genetics Interleukin-6 / genetics Interleukin-8 / genetics Lipopolysaccharides / pharmacology Oleic Acid / pharmacology Poly I-C / pharmacology RNA, Messenger / metabolism Real-Time Polymerase Chain Reaction Tissue Donors Tumor Necrosis Factor-alpha / genetics alpha-Linolenic Acid / pharmacology* |
| Chemical | |
Reg. No./Substance:
|
0/Anti-Inflammatory Agents, Non-Steroidal; 0/I-kappa B Proteins; 0/IL6 protein, human; 0/Interleukin-1beta; 0/Interleukin-6; 0/Interleukin-8; 0/Lipopolysaccharides; 0/RNA, Messenger; 0/Tumor Necrosis Factor-alpha; 112-80-1/Oleic Acid; 139874-52-5/NF-kappaB inhibitor alpha; 24939-03-5/Poly I-C; 463-40-1/alpha-Linolenic Acid; 50-02-2/Dexamethasone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Circadian and wake-dependent effects on the pupil light reflex in response to narrow-bandwidth light...
Next Document: Characterization of fusarium keratitis outbreak isolates: contribution of biofilms to antimicrobial ...