Document Detail

Anti-inflammatory effects of alpha linolenic acid on human corneal epithelial cells.
MedLine Citation:
PMID:  22669722     Owner:  NLM     Status:  MEDLINE    
PURPOSE: Systemic polyunsaturated fatty acids (PUFAs) were shown to improve the symptoms of dry eye syndrome due to their anti-inflammatory effects. This study evaluated the in vitro anti-inflammatory effects of PUFAs on human corneal epithelial (HCE) cells.
METHODS: HCE cells were incubated for 2 hours with different concentrations of PUFAs: alpha-linolenic acid (ALA), gamma-linolenic acid (GLA), and linoleic acid (LA). Oleic acid (OA) and dexamethasone (DM) served as negative and positive controls, respectively. Cells were stimulated with either polyinosinic:polycytidylic acid (poly I:C) or lipopolysaccharide (LPS) complex. The protein contents and mRNA expression levels of IL-6, IL-8, IL-1β, and TNF-α were evaluated with multiplex fluorescent bead immunoassay and real-time PCR, respectively. The expression of inhibitory factor-κBα (I-κBα) was evaluated with real-time PCR.
RESULTS: The protein and mRNA levels of IL-6, IL-8, IL-1β, and TNF-α were significantly increased after stimulation with LPS or poly I:C. Following treatment with ALA, a significant decrease was demonstrated in the protein content of TNF-α to 23.81% (P < 0.001), IL-6 to 46.71% (P < 0.001), IL-1β to 20.86% (P < 0.05), and IL-8 to 52.21% (P < 0.001). Similar results were demonstrated at the mRNA level. The anti-inflammatory effects of ALA were similar to those of DM for all of the pro-inflammatory cytokines. The ALA inhibition of the pro-inflammatory cytokines was associated with a significant reduction of I-κBα.
CONCLUSIONS: ALA may serve as a potent anti-inflammatory agent in ocular surface inflammation. The anti-inflammatory effects of ALA are comparable to those of corticosteroids, and are mediated through NF-κB signal transduction.
Nir Erdinest; Or Shmueli; Yoni Grossman; Haim Ovadia; Abraham Solomon
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-07-03
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  53     ISSN:  1552-5783     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-07-04     Completed Date:  2012-10-16     Revised Date:  2013-01-09    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4396-406     Citation Subset:  IM    
Department of Ophthalmology, The Agnes Ginges Center for Human Neurogenetics, Hadassah-Hebrew University Medical Center, Jerusalem, Israel.
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MeSH Terms
Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
Blotting, Western
Cell Survival
Cells, Cultured
Dexamethasone / pharmacology
Epithelium, Corneal / drug effects*,  metabolism
Fluorescent Antibody Technique
I-kappa B Proteins / genetics
Interleukin-1beta / genetics
Interleukin-6 / genetics
Interleukin-8 / genetics
Lipopolysaccharides / pharmacology
Oleic Acid / pharmacology
Poly I-C / pharmacology
RNA, Messenger / metabolism
Real-Time Polymerase Chain Reaction
Tissue Donors
Tumor Necrosis Factor-alpha / genetics
alpha-Linolenic Acid / pharmacology*
Reg. No./Substance:
0/Anti-Inflammatory Agents, Non-Steroidal; 0/I-kappa B Proteins; 0/IL6 protein, human; 0/Interleukin-1beta; 0/Interleukin-6; 0/Interleukin-8; 0/Lipopolysaccharides; 0/RNA, Messenger; 0/Tumor Necrosis Factor-alpha; 112-80-1/Oleic Acid; 139874-52-5/NF-kappaB inhibitor alpha; 24939-03-5/Poly I-C; 463-40-1/alpha-Linolenic Acid; 50-02-2/Dexamethasone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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