| Anti-idiotypic antibodies of a predefined specificity generated against CDR3VH synthetic peptides define a private anti-CD4 idiotype. | |
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MedLine Citation:
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PMID: 2116595 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A synthetic peptide corresponding to the third complementarity determining region (CDR) of the heavy chain (CDR3VH) of anti-Leu3a, a monoclonal anti-CD4 antibody which inhibits HIV gp120 binding to CD4, was used to elicit specific anti-peptide antibodies in rabbits. The anti-peptide antisera showed anti-idiotypic antibody (anti-Id) activity and recognized both the immunizing peptide and the intact cognate protein by ELISA. In addition, the antisera reacted with isolated heavy chains of anti-Leu3a by Western blot analysis. The lack of reactivity with a panel of monoclonal anti-CD4 antibodies suggested that the anti-peptide antisera recognize a private idiotype (Id) associated with the anti-Leu3a CDR3VH region. Further studies demonstrated the inability of the rabbit antisera to inhibit the binding of anti-Leu3a to the CD4 molecule. In addition, soluble recombinant CD4 was unable to inhibit the binding of the rabbit anti-peptide antisera to anti-Leu3a indicating that the CDR3VH region may not be involved in CD4 recognition. Anti-Id containing sera from mice, rabbits and nonhuman primates immunized with the intact anti-Leu3a molecule did not bind the CDR3VH synthetic peptide, suggesting that the corresponding region of anti-Leu3a may not represent an immunodominant idiotypic determinant in thes e species. These results suggest the potential use of synthetic peptides corresponding to immunoglobulin variable (V) region amino acid sequences in generating anti-Id reagents of a predefined specificity. In addition, V-region synthetic peptides may be useful in mapping the idiotopes recognized by an anti-Id response to the cognate molecule. |
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Authors:
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R Attanasio; R C Kennedy; J S Allan; V C Maino; D Buck; P Kanda |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Molecular immunology Volume: 27 ISSN: 0161-5890 ISO Abbreviation: Mol. Immunol. Publication Date: 1990 Jun |
Date Detail:
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Created Date: 1990-09-07 Completed Date: 1990-09-07 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7905289 Medline TA: Mol Immunol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 513-22 Citation Subset: IM; X |
Affiliation:
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Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, TX 78284. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Antibodies, Anti-Idiotypic / immunology* Antibodies, Monoclonal / immunology Antibody Specificity Antigens, CD4 / immunology* Blotting, Western Immunoglobulin Heavy Chains / immunology Immunoglobulin Idiotypes* Immunoglobulin Variable Region / immunology Molecular Sequence Data Peptides / immunology Rabbits |
| Grant Support | |
ID/Acronym/Agency:
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AI26492/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Anti-Idiotypic; 0/Antibodies, Monoclonal; 0/Antigens, CD4; 0/Immunoglobulin Heavy Chains; 0/Immunoglobulin Idiotypes; 0/Immunoglobulin Variable Region; 0/Peptides |
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