Document Detail


Anti-human very late antigen-alpha4 (CD49d) monoclonal antibody (BU49) cross-reacts with the canine B-cell leukemia cell line GL-1, resulting in the induction of homotypic cell aggregation.
MedLine Citation:
PMID:  20304388     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have found that the anti-human very late antigen-alpha4 (VLA-alpha4) (CD49d) monoclonal antibody (mAb) BU49 cross-reacts with the canine B-cell leukemia cell line GL-1. Interestingly, the BU49 mAb specifically induced the homotypic cell aggregation of GL-1 cells accompanied by morphological changes. Homotypic cell aggregates induced by BU49 mAb were blocked by the presence of a protein kinase C inhibitor, a protein kinase A inhibitor, an actin filament formation inhibitor, and an EDTA. On the other hand, a protein tyrosine kinase inhibitor, a DNA-synthesis inhibitor, and an anti-canine CD45 mAb did not affect the GL-1 homotypic cell aggregation induced by BU49 mAb. The BU49 mAb immunoprecipitated at a molecular weight of about 150kDa in the GL-1 cells, similar to the results in the human monocyte-like cell line U937. Taken together, our results provide the first evidence that human CD49d recognized by BU49 mAb has unique immunological functions against canine cells.
Authors:
Nobunao Ikewaki; Munekazu Nakaichi; Takuya Mizuno; Norito Takamura; Jin Tokunaga; Kenji Ogata; Hidetoshi Inoko; Ryuichi Otsu
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Publication Detail:
Type:  Journal Article     Date:  2010-02-26
Journal Detail:
Title:  Cellular immunology     Volume:  263     ISSN:  1090-2163     ISO Abbreviation:  Cell. Immunol.     Publication Date:  2010  
Date Detail:
Created Date:  2010-05-03     Completed Date:  2010-08-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1246405     Medline TA:  Cell Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  55-64     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Inc. All rights reserved.
Affiliation:
Department of Animal Pharmaceutical Science, Kyushu University of Health and Welfare School of Pharmaceutical Sciences, and Institute of Immunology, Takahashi Educational Institute, 1714-1 Yoshino-cho, Nobeoka, Miyazaki 882-8508, Japan. nikewaki@phoenix.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / immunology*
Cell Aggregation / immunology*
Cell Line, Tumor
Cyclic AMP-Dependent Protein Kinases / immunology,  metabolism
Dogs
Edetic Acid / pharmacology
Humans
Integrin alpha4beta1 / immunology*
Leukemia, B-Cell / immunology*
Leukocytes, Mononuclear / drug effects*,  immunology,  metabolism,  pathology
Male
Microfilaments / immunology
Protein Kinase C / immunology,  metabolism
U937 Cells
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Integrin alpha4beta1; 60-00-4/Edetic Acid; EC 2.7.11.11/Cyclic AMP-Dependent Protein Kinases; EC 2.7.11.13/Protein Kinase C

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