| Anti-human immunodeficiency virus type 1 activity of phosphorothioate analogs of oligodeoxynucleotides: penetration and localization of oligodeoxynucleotides in HIV-1-infected MOLT-4 cells. | |
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MedLine Citation:
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PMID: 7528375 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Phosphorothioate antisense oligodeoxynucleotide against HIV-1 rev (S-ODN-rev) inhibits virus-induced cytopathic effects (CPE) in acute infection and inhibits the expression of HIV-1 core protein, p24, in chronically infected cells in vitro. HIV-1 reverse transcriptase activity was not affected by S-ODN-rev at the high concentrations of 5-25 microM, which were 250-1250 times higher than the concentration required to achieve 100% HIV-1-induced CPE inhibition. [32P]-labeled S-ODN-rev was rapidly uptaken by MOLT-4 cells, whereas [32P]-SO-ODN-rev and [32P]-O-ODN-rev were not. In the observation of FITC-S-ODN-rev-treated MOLT-4 cells by a confocal laser scanning microscope, diffuse fluorescence was apparently observed in the cytoplasm. Interestingly, fluorescence signals were accumulated in the nuclear region of chronically infected MOLT-4/HIV-1 cells 60 min after incubation. FITC-labeled homooligomer, FITC-S-dC20 and FIT-C-S-dT20, also accumulated in the nucleus of MOLT-4/HIV-1 cells, but weak fluorescence was observed on the cell membrane and in the cytoplasm of the FITC-S-random treated MOLT-4/HIV-1 and MOLT-4 cells. |
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Authors:
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H Nakashima; Y Shoji; S G Kim; J Shimada; Y Mizushima; M Ito; N Yamamoto; H Takaku |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Nucleic acids research Volume: 22 ISSN: 0305-1048 ISO Abbreviation: Nucleic Acids Res. Publication Date: 1994 Nov |
Date Detail:
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Created Date: 1995-01-20 Completed Date: 1995-01-20 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0411011 Medline TA: Nucleic Acids Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 5004-10 Citation Subset: IM; X |
Affiliation:
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Department of Microbiology, Yamanashi Medical University, Japan. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line, Transformed Cell Nucleus / metabolism Cytopathogenic Effect, Viral / drug effects Cytoplasm / metabolism Genes, rev / genetics HIV Core Protein p24 / analysis HIV Reverse Transcriptase HIV-1 / drug effects*, genetics Humans Molecular Sequence Data Oligonucleotides, Antisense / chemical synthesis, pharmacology* RNA-Directed DNA Polymerase / metabolism T-Lymphocytes / virology Thionucleotides / chemical synthesis, pharmacology* Virus Replication / drug effects |
| Chemical | |
Reg. No./Substance:
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0/HIV Core Protein p24; 0/Oligonucleotides, Antisense; 0/Thionucleotides; EC 2.7.7.49/HIV Reverse Transcriptase; EC 2.7.7.49/RNA-Directed DNA Polymerase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): Nucleic Acids Res ISSN: 0305-1048 ISSN: 1362-4962 |
Article Information Download PDF  Print publication date: Day: 25 Month: 11 Year: 1994 Volume: 22 Issue: 23 First Page: 5004 Last Page: 5010 ID: 523770 PubMed Id: 7528375 |
| Anti-human immunodeficiency virus type 1 activity of phosphorothioate analogs of oligodeoxynucleotides: penetration and localization of oligodeoxynucleotides in HIV-1-infected MOLT-4 cells. | |
| H Nakashima | |
| Y Shoji | |
| S G Kim | |
| J Shimada | |
| Y Mizushima | |
| M Ito | |
| N Yamamoto | |
| H Takaku | |
| Department of Microbiology, Yamanashi Medical University, Japan. |
|
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