Document Detail


Anti-herpes simplex virus activity of moronic acid purified from Rhus javanica in vitro and in vivo.
MedLine Citation:
PMID:  10086989     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rhus javanica, a medicinal herb, has been shown to exhibit oral therapeutic anti-herpes simplex virus (HSV) activity in mice. We purified two major anti-HSV compounds, moronic acid and betulonic acid, from the herbal extract by extraction with ethyl acetate at pH 10 followed by chromatographic separations and examined their anti-HSV activity in vitro and in vivo. Moronic acid was quantitatively a major anti-HSV compound in the ethyl acetate-soluble fraction. The effective concentrations for 50% plaque reduction of moronic acid and betulonic acid for wild-type HSV type 1 (HSV-1) were 3.9 and 2.6 microgram/ml, respectively. The therapeutic index of moronic acid (10.3-16.3) was larger than that of betulonic acid (6.2). Susceptibility of acyclovir-phosphonoacetic acid-resistant HSV-1, thymidine kinase-deficient HSV-1, and wild-type HSV type 2 to moronic acid was similar to that of the wild-type HSV-1. When this compound was administered orally to mice infected cutaneously with HSV-1 three times daily, it significantly retarded the development of skin lesions and/or prolonged the mean survival times of infected mice without toxicity compared with the control. Moronic acid suppressed virus yields in the brain more efficiently than those in the skin. This was consistent with the prolongation of mean survival times. Thus, moronic acid was purified as a major anti-HSV compound from the herbal extract of Rhus javanica. Mode of the anti-HSV activity was different from that of ACV. Moronic acid showed oral therapeutic efficacy in HSV-infected mice and possessed novel anti-HSV activity that was consistent with that of the extract.
Authors:
M Kurokawa; P Basnet; M Ohsugi; T Hozumi; S Kadota; T Namba; T Kawana; K Shiraki
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of pharmacology and experimental therapeutics     Volume:  289     ISSN:  0022-3565     ISO Abbreviation:  J. Pharmacol. Exp. Ther.     Publication Date:  1999 Apr 
Date Detail:
Created Date:  1999-04-21     Completed Date:  1999-04-21     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0376362     Medline TA:  J Pharmacol Exp Ther     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  72-8     Citation Subset:  IM    
Affiliation:
Department of Virology, Toyama Medical and Pharmaceutical University, Sugitani, Toyama, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antiviral Agents / isolation & purification,  pharmacology*
Brain / drug effects,  virology
Ethanol
Herpesviridae Infections / drug therapy,  virology
Herpesvirus 1, Human / drug effects*
Mice
Mice, Inbred BALB C
Oleanolic Acid / analogs & derivatives*,  isolation & purification,  pharmacology
Plant Extracts / chemistry
Plants, Toxic*
Plaque Assay
Skin / drug effects,  virology
Solvents
Toxicodendron / chemistry*
Chemical
Reg. No./Substance:
0/Antiviral Agents; 0/Plant Extracts; 0/Solvents; 0/betulonic acid; 508-02-1/Oleanolic Acid; 64-17-5/Ethanol; 6713-27-5/moronic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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