| Anti-cancer effect of resveratrol is associated with induction of apoptosis via a mitochondrial pathway alignment. | |
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MedLine Citation:
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PMID: 18290328 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Resveratrol, a phytoalexin found in the skin of grapes, is believed to have multiple bioactivities including anti-cancer, anti-carcinogenesis and antiinflammatory. The mechanisms by which resveratrol might produce these effects are not well understood. In this study, malignant human pancreatic cancer cells were treated without or with resveratrol in combination with ionizing radiation (IR), and then the mitochondrial function of treated cells was evaluated using several standardized assays. They include the Calcein AM method for mitochondria transition pore; the JC-1 staining method for mitochondria membrane potential; the CM-H2DCFDA method for reactive oxygen species; and the Annexin V/propidium iodide (PI) method for apoptosis/cell death. Our results indicated that (1) pore function was partially intact after resveratrol, but resveratrol probably interfered with the accumulation of intracellular Calcein AM; (2) depolarization of the mitochondria membrane was increased in the resveratrol treated cells, consistent with mitochondrial dysfunction; (3) ROS was slightly increased with resveratrol, a phenomenon that was greatly increased when this agent was combined with IR; and (4) in parallel with the above changes in mitochondrial and drug transport, cells treated with resveratrol showed increased apoptosis as measured by Annexin V/PI staining. In summary, the anti-cancer effect of resveratrol is associated with the damage of mitochondrial function that leads to increased ROS, apoptosis, and possibly intracellular drug accumulation via inhibition of proteins involved in multi-drug resistance (MDR). |
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Authors:
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Weimin Sun; Wei Wang; Jung Kim; Peter Keng; Shanmin Yang; Hengshan Zhang; Chaomei Liu; Paul Okunieff; Lurong Zhang |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Advances in experimental medicine and biology Volume: 614 ISSN: 0065-2598 ISO Abbreviation: Adv. Exp. Med. Biol. Publication Date: 2008 |
Date Detail:
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Created Date: 2008-02-22 Completed Date: 2008-04-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0121103 Medline TA: Adv Exp Med Biol Country: United States |
Other Details:
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Languages: eng Pagination: 179-86 Citation Subset: IM |
Affiliation:
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Department of Radiation Oncology, University of Rochester Medical Center, Rochester, NY 14642, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anticarcinogenic Agents
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pharmacology* Apoptosis / drug effects*, physiology Benzimidazoles / metabolism Carbocyanines / metabolism Cell Death / drug effects Cell Line, Tumor Dose-Response Relationship, Drug Fluoresceins / analysis, metabolism Fluorescent Dyes Humans Membrane Potentials / drug effects Mitochondria / metabolism* Mitochondrial Membranes / drug effects, metabolism Pancreatic Neoplasms / metabolism, pathology Reactive Oxygen Species / analysis, metabolism Stilbenes / pharmacology* |
| Grant Support | |
ID/Acronym/Agency:
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U19-AI067733/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Anticarcinogenic Agents; 0/Benzimidazoles; 0/Carbocyanines; 0/Fluoresceins; 0/Fluorescent Dyes; 0/Reactive Oxygen Species; 0/Stilbenes; 148504-34-1/calcein AM; 21527-78-6/5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine; 501-36-0/resveratrol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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