| Anti-bacterial and anti-toxic immunity induced by a killed whole-cell-cholera toxin B subunit cholera vaccine is essential for protection against lethal bacterial infection in mouse pulmonary cholera model. | |
| | |
MedLine Citation:
|
PMID: 23187318 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
The lack of appropriate animal model for studying protective immunity has limited vaccine development against cholera. Here, we demonstrate a pulmonary cholera model conferred by intranasal administration of mice with live Vibrio cholerae. The bacterial components, but not cholera toxin, caused lethal and acute pneumonia by inducing massive inflammation. Intranasal immunization with Dukoral, comprising killed whole bacteria and recombinant cholera toxin B subunit (rCTB), developed both mucosal and systemic antibody responses with protection against the lethal challenge. Either rCTB-free Dukoral or rCTB alone partially protected the mice against the challenge. However, reconstitution of rCTB-free Dukoral with rCTB restored full protection. Parenteral immunization with Dukoral evoked strong systemic immunity without induction of mucosal immunity or protection from the challenge. These results suggest that both anti-bacterial and anti-toxic immunity are required for protection against V. cholerae-induced pneumonia, and this animal model is useful for pre-clinical evaluation of candidate cholera vaccines.Mucosal Immunology advance online publication 28 November 2012; doi:10.1038/mi.2012.121. |
| | |
Authors:
|
S-S Kang; J S Yang; K W Kim; C-H Yun; J Holmgren; C Czerkinsky; S H Han |
Related Documents
:
|
23685068 - The prospects and challenges of universal vaccines for influenza. 23316448 - Strategies for intranasal delivery of vaccines. 23618628 - The effect of vaccines based on ovalbumin coupled to gas-filled microbubbles for reduci... 23419518 - Influenza diagnosis and vaccination in poland. 9342258 - Hiv-1 risk and vaccine acceptability in the ugandan military. 23616838 - Utilizing an electronic health record system to improve vaccination coverage in children. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-11-28 |
Journal Detail:
|
Title: Mucosal immunology Volume: - ISSN: 1935-3456 ISO Abbreviation: Mucosal Immunol Publication Date: 2012 Nov |
Date Detail:
|
Created Date: 2012-11-28 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101299742 Medline TA: Mucosal Immunol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Laboratory Sciences Division, International Vaccine Institute, Seoul, Republic of Korea. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The adult penile urethra is a novel entry site for HIV-1 that preferentially targets resident urethr...
Next Document: Variability of blood lactate during and between given constant prolonged cycling power.