Document Detail

Anti-apoptotic role of phospholipase D isozymes in the glutamate-induced cell death.
MedLine Citation:
PMID:  12642902     Owner:  NLM     Status:  MEDLINE    
Phospholipase D (PLD) plays an important role as an effector in a variety of physiological processes that reveal it to be a member of the signal transducing phospholipases. Recently, PLD2 was reported as a necessary intermediate in preventing apoptosis induced by hydrogen peroxide or hypoxia in rat pheochromocytoma (PC12) cells. The data presented here show that both PLD isozymes, PLD1 and PLD2 are also required in attenuating glutamate-induced cell death in PC12 cells. Treatment of PC12 cells with glutamate resulted in induction of apoptosis in these cells, which is accompanied by decreased PLD activity and increased ceramide concentration. Incubation of PC12 cells with exogenous C6-ceramide showed a time-dependent decrease of PLD activity. When cDNAs of PLD1 and PLD2 were transfected into PC12 cells respectively, overexpression of PLD1 or PLD2 resulted in inhibition of glutamate-induced apoptotic cell death. These data indicate that both PLD1 and PLD2 play a protective role against glutamate-induced cell death in PC12 cells.
Kyung-Ok Kim; Kweon-Haeng Lee; Young-Hoon Kim; Seung-Kiel Park; Joong-Soo Han
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental & molecular medicine     Volume:  35     ISSN:  1226-3613     ISO Abbreviation:  Exp. Mol. Med.     Publication Date:  2003 Feb 
Date Detail:
Created Date:  2003-03-18     Completed Date:  2003-10-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9607880     Medline TA:  Exp Mol Med     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  38-45     Citation Subset:  IM    
Institute of Biomedical Science and Department of Biochemistry, College of Medicine, Hanyang University, Seoul 133-791, Korea.
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MeSH Terms
Apoptosis / drug effects,  physiology*
Cell Survival / drug effects
Ceramides / pharmacology
Dose-Response Relationship, Drug
Enzyme Activation
Gene Expression Regulation, Enzymologic / drug effects
Glutamic Acid / toxicity*
Isoenzymes / drug effects,  genetics,  metabolism*
PC12 Cells
Phospholipase D / chemistry,  drug effects,  genetics,  metabolism*
Sphingolipids / metabolism
Reg. No./Substance:
0/Ceramides; 0/Isoenzymes; 0/N-caproylsphingosine; 0/Sphingolipids; 56-86-0/Glutamic Acid; EC D

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