Document Detail


Anti-apoptotic protection afforded by cardioplegic Celsior and Histidine buffer solutions to hearts subjected to ischemia and ischemia/reperfusion.
MedLine Citation:
PMID:  21866562     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Cardiomyocytes undergo apoptosis in response to ischemia and ischemia/reperfusion (I/R). During heart preservation, inhibition of apoptosis is critical to avoid organ failure. We aimed to compare the protection afforded by Celsior (Cs) and Histidine-buffer solution (HBS) against apoptotic signaling in hearts subjected to moderate (4h) vs. severe (6h) ischemia alone or followed by 30 min reperfusion. The impact of gender on cardioplegic protection was also explored. Hearts from male and female Wistar-Han rats were divided by gender in distinct groups: control, perfusion_control, ischemia and I/R. Ischemia and I/R groups were divided in subgroups Cs or HBS, and subjected to 4 or 6h ischemia alone or followed by reperfusion. Proteins involved in apoptotic signaling (p53, Bax, Fas, FasL and Flip) were quantified by western-blot in mitochondria and/or whole tissue. Caspase-3, 8 and 9-like activities were measured and hemodynamic parameters were monitored. Ischemia activated p53/Bax signaling. After I/R, HBS-preserved hearts had lower p53/Bax content in mitochondrial fractions than Cs-preserved hearts. The p53/Bax decrease in tissue samples was mostly observed in females. Caspase 3-like activity was increased in HBS-preserved male hearts. The heart rate was decreased in Cs and HBS-preserved hearts. Fas protein levels remained unaltered in all conditions but soluble FasL increased from 4 to 6h preservation in Cs and HBS. Hearts from male rats were more prone to apoptosis and myocardial dysfunction. HBS and Cs were not effective in inhibiting apoptotic signaling although HBS presented best overall results. Both solutions should be improved to prevent apoptosis and myocardial dysfunction after I/R. J. Cell. Biochem. © 2011 Wiley-Liss, Inc.
Authors:
Marco G Alves; Nuno G Machado; Vilma A Sardão; Rui A Carvalho; Paulo J Oliveira
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-8-22
Journal Detail:
Title:  Journal of cellular biochemistry     Volume:  -     ISSN:  1097-4644     ISO Abbreviation:  -     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-8-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8205768     Medline TA:  J Cell Biochem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Wiley-Liss, Inc.
Affiliation:
CNC-Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra - Portugal; Department of Life Sciences, Faculty of Science and Technology, University of Coimbra, 3004-517 Coimbra - Portugal.
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