| Anti-apoptotic activity and proteasome-mediated degradation of Xenopus Mcl-1 protein in egg extracts. | |
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MedLine Citation:
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PMID: 21454490 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Xenopus egg extracts execute spontaneous apoptosis without the requirement of transcription and translation, and this intrinsic mechanism is supposed to be involved in the physiological elimination of aged eggs. Although apoptosis in this system is carried out by maternally stockpiled materials, the endogenous apoptosis regulators present in egg extracts are still poorly characterized. Here we examined the mRNA expression profiles and apoptosis-regulating functions of 13 Xenopus Bcl-2 family proteins in egg extracts. Among these, we found that endogenous Xenopus Mcl-1 (xMcl-1) physiologically inhibited apoptosis by counteracting the pro-apoptotic activity of endogenous Xenopus Bid in egg extracts. Exogenously added recombinant xMcl-1 was rapidly degraded by proteasome in egg extracts, and we identified the destabilizing region in the N terminus of xMcl-1. Our results suggest that the proteolytic decay of xMcl-1 may change the functional balance between pro- and anti-apoptotic activities of Bcl-2 family proteins, thereby regulating the timing of cytochrome c release in egg extracts. |
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Authors:
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Yuichi Tsuchiya; Shigeru Yamashita |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-03-17 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 286 ISSN: 1083-351X ISO Abbreviation: J. Biol. Chem. Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-05-02 Completed Date: 2011-07-01 Revised Date: 2012-05-08 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 15806-14 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Toho University School of Medicine, Tokyo, Japan. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AB427203; AB427204 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Cell-Free System / metabolism Female Molecular Sequence Data Oocytes / metabolism* Proteasome Endopeptidase Complex / genetics, metabolism* Proto-Oncogene Proteins c-bcl-2 / genetics, metabolism* RNA, Messenger / genetics, metabolism Xenopus Proteins / genetics, metabolism* Xenopus laevis |
| Chemical | |
Reg. No./Substance:
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0/Proto-Oncogene Proteins c-bcl-2; 0/RNA, Messenger; 0/Xenopus Proteins; EC 3.4.25.1/Proteasome Endopeptidase Complex |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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