| Anti-apoptotic activity of Bak Foong Pills and its ingredients on 6-hydroxydopamine-induced neurotoxicity in PC12 cells. | |
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MedLine Citation:
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PMID: 16154779 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bak Foong Pills (BFP), a traditional Chinese medicine used for centuries for the enhancement of women's health, was shown to display neuro-protective activity in the 1-methyl-4-phenyl-1,2,4,6,-tetrahydro-pyridine (MPTP)-induced mouse model in a previous study. In order to elucidate its mechanism of action, we investigated the anti-apoptotic properties of Bak Foong Pills and its main ingredients, including Panax ginseng, Angelica sinensis, Glycyrrhiza uralensis, and Ligusticum chuanxiong, in the 6-hydroxydopamine (6-OHDA)-treated PC12 cell model. The addition of the neurotoxin could cause significant cell death and reduction of cell proliferation, as shown in the results determined by MTT assay, nitric oxide (NO) measurement and flow cytometric propidium iodine (PI) staining analysis, while pre-treatment of PC12 cell with either BFP or its main ingredients prevented the toxicity to some degree. In addition, the neurotoxin caused an elevated activation of caspase-3, the key enzyme for activation of the cellular apoptotic cascade, whereas BFP or its main ingredients inhibited the activation of caspase-3. These results strongly indicate that BFP and its main ingredients may provide a useful therapeutic strategy for the treatment of neurodegenerative diseases, such as Parkinson's disease. |
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Authors:
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Rui Rui Jia; Yu Lin Gou; Lok Sze Ho; Chuen-Pei Ng; Ning Hua Tan; Hsiao Chang Chan |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell biology international Volume: 29 ISSN: 1065-6995 ISO Abbreviation: Cell Biol. Int. Publication Date: 2005 Oct |
Date Detail:
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Created Date: 2005-09-27 Completed Date: 2006-02-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9307129 Medline TA: Cell Biol Int Country: England |
Other Details:
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Languages: eng Pagination: 835-42 Citation Subset: IM |
Affiliation:
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Epithelial Cell Biology Research Center, Department of Physiology, The Chinese University of Hong Kong, Shatin, Hong Kong, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects* Caspase 3 Caspases / drug effects, metabolism Cell Proliferation / drug effects Cell Survival / drug effects DNA Damage / drug effects Dose-Response Relationship, Drug Drugs, Chinese Herbal / pharmacology* Kinetics Neurons / drug effects* Neuroprotective Agents / pharmacology* Neurotoxins / antagonists & inhibitors*, toxicity Nitric Oxide / antagonists & inhibitors, metabolism Oxidopamine / antagonists & inhibitors*, toxicity PC12 Cells Rats |
| Chemical | |
Reg. No./Substance:
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0/Bak Foong Pills; 0/Drugs, Chinese Herbal; 0/Neuroprotective Agents; 0/Neurotoxins; 10102-43-9/Nitric Oxide; 1199-18-4/Oxidopamine; EC 3.4.22.-/Casp3 protein, mouse; EC 3.4.22.-/Casp3 protein, rat; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases |
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