Document Detail


Anti-angiogenic and anti-metastatic effects of beta-1,3-D-glucan purified from Hanabiratake, Sparassis crispa.
MedLine Citation:
PMID:  19182386     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sparassis crispa (SC), Hanabiratake in Japanese, is an edible mushroom with medicinal properties, that contains more than 40% beta-D-glucan. It was concluded from results of the methylation study that beta-D-glucan from SC (SBG) was composed of a backbone of beta-(1-->3)-linked D-glucopyranosyl residues, and had beta-D-glucopyranosyl groups joined through O-6 and O-2 of D-glucose of the backbone. We purified SBG and investigated its anti-angiogenic functions and anti-metastatic effects on neoplasm using different animal models. The oral administration of the purified SBG suppressed B16-F10 cell-induced angiogenesis in the dorsal air sac assay using female ICR mice as well as vascular endothelial growth factor induced neovascularization in the Matrigel plug assay using female C57BL/6J mice. Furthermore, it suppressed the growth and numbers of the metastatic tumor foci in lung, along with the primary tumor growth in the spontaneous metastatic model using female C57BL/6J mice. From these results, it is apparent that the oral administration of SBG results in suppressive effect on tumor growth and metastasis in lung through the inhibition of tumor induced-angiogenesis. These effects are not a result of direct action on the endothelial cells because cell growth, migration and capillary-like tube formation were not affected in the human umbilical vein endothelial cells by SBG application. This is the first report showing that the oral administration of SBG is capable of suppressing angiogenesis and metastasis.
Authors:
Kyosuke Yamamoto; Takashi Kimura; Akio Sugitachi; Nariaki Matsuura
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biological & pharmaceutical bulletin     Volume:  32     ISSN:  0918-6158     ISO Abbreviation:  Biol. Pharm. Bull.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-02-02     Completed Date:  2009-03-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9311984     Medline TA:  Biol Pharm Bull     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  259-63     Citation Subset:  IM    
Affiliation:
Research & Development Center, Unitika Ltd., Uji, Kyoto, Japan. kyosuke-yamamoto@unitika.co.jp
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MeSH Terms
Descriptor/Qualifier:
Agaricales / chemistry*
Angiogenesis Inhibitors / isolation & purification,  pharmacology*
Animals
Antineoplastic Agents / isolation & purification,  pharmacology*
Capillaries / drug effects,  pathology
Cell Line
Cell Movement / drug effects
Cell Proliferation / drug effects
Female
Humans
Lung Neoplasms / prevention & control,  secondary
Melanoma, Experimental / pathology
Methylation
Mice
Mice, Inbred ICR
Neoplasm Metastasis / prevention & control
Neoplasm Transplantation
Neovascularization, Pathologic / pathology,  prevention & control
Structure-Activity Relationship
Vascular Endothelial Growth Factor A / pharmacology
beta-Glucans / isolation & purification,  pharmacology*
Chemical
Reg. No./Substance:
0/Angiogenesis Inhibitors; 0/Antineoplastic Agents; 0/Vascular Endothelial Growth Factor A; 0/beta-1,3-D-glucan; 0/beta-Glucans

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