Document Detail


Anti-adipogenic effect of mulberry leaf ethanol extract in 3T3-L1 adipocytes.
MedLine Citation:
PMID:  25489399     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
BACKGROUND/OBJECTIVES: Adipogenesis is part of the cell differentiation process in which undifferentiated fibroblasts (pre-adipocytes) become mature adipocytes with the accumulation of lipid droplets and subsequent cell morphological changes. Several transcription factors and food components have been suggested to be involved in adipogenesis. The aim of this study was to determine whether mulberry leaf ethanol extract (MLEE) affects adipogenesis in 3T3-L1 adipocytes.
MATERIALS/METHODS: The 3T3-L1 adipocytes were treated with different doses of MLEE for 8 days starting 2 days post-confluence. Cell viability, fat accumulation, and adipogenesis-related factors including CCAAT-enhancer-binding protein alpha (C/EBPα), peroxisome proliferator-activated receptor gamma (PPARγ), PPARγ coactivator 1 alpha (PGC-1α), fatty acid synthase (FAS), and adiponectin were analyzed.
RESULTS: Results showed that MLEE treatments at 10, 25, 50, and 100 µg/ml had no effect on cell morphology and viability. Without evident toxicity, all MLEE treated cells had lower fat accumulation compared with control as shown by lower absorbances of Oil Red O stain. MLEE at 50 and 100 µg/ml significantly reduced protein levels of PPARγ, PGC-1α, FAS, and adiponectin in differentiated adipocytes. Furthermore, protein level of C/EBPα was significantly decreased by the treatment of 100 µg/ml MLEE.
CONCLUSION: These results demonstrate that MLEE treatment has an anti-adipogenic effect in differentiated adipocytes without toxicity, suggesting its potential as an anti-obesity therapeutic.
Authors:
Soo Jin Yang; Na-Young Park; Yunsook Lim
Related Documents :
22417809 - Proteomic analysis of acquired tamoxifen resistance in mcf-7 cells reveals expression s...
20614479 - Interstitial cells of cajal, the maestro in health and disease.
12419659 - Possible role of contact following in the generation of coherent motion of dictyosteliu...
15661309 - A simple method to study cellular migration.
10962479 - Redundancy of lamellipodia in locomoting walker carcinosarcoma cells.
21539399 - Polydopamine/liposome coatings and their interaction with myoblast cells.
19207679 - Positive effect of silymarin on cell growth and differentiation in bovine and murine ma...
24280069 - Curcumin glucuronides: assessing the proliferative activity against human cell lines.
11959829 - Mosaic analyses reveal the function of drosophila ras in embryonic dorsoventral pattern...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-11-28
Journal Detail:
Title:  Nutrition research and practice     Volume:  8     ISSN:  1976-1457     ISO Abbreviation:  Nutr Res Pract     Publication Date:  2014 Dec 
Date Detail:
Created Date:  2014-12-9     Completed Date:  -     Revised Date:  2014-12-10    
Medline Journal Info:
Nlm Unique ID:  101311052     Medline TA:  Nutr Res Pract     Country:  -    
Other Details:
Languages:  ENG     Pagination:  613-617     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The Comprehensive AOCMF Classification System: Fracture Case Collection, Diagnostic Imaging Work Up,...
Next Document:  Antioxidant action of soy isoflavones on oxidative stress and antioxidant enzyme activities in exerc...