| Anti-U1 RNP antibodies in cerebrospinal fluid are associated with central neuropsychiatric manifestations in systemic lupus erythematosus and mixed connective tissue disease. | |
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MedLine Citation:
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PMID: 20722023 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To determine the significance of anti-U1 RNP antibodies in the cerebrospinal fluid (CSF) of patients with systemic lupus erythematosus (SLE) or mixed connective tissue disease (MCTD) who have central neuropsychiatric SLE (NPSLE). METHODS: The frequency of antinuclear antibodies including anti-U1 RNP antibodies in the sera and CSF of 24 patients with SLE and 4 patients with MCTD, all of whom had neuropsychiatric syndromes, was determined using an RNA immunoprecipitation assay and an enzyme-linked immunosorbent assay. The frequency of anti-U1 RNP antibodies in the CSF of patients with central NPSLE was examined, and the anti-U1 RNP index ([CSF anti-U1 RNP antibodies/serum anti-U1 RNP antibodies]/[CSF IgG/serum IgG]) was compared with CSF interleukin-6 (IL-6) levels and the albumin quotient (Qalb, an indicator of blood-brain barrier damage). CSF and serum antibodies against U1-70K, U1-A, and U1-C, including autoantigenic regions, were examined, and the U1-70K, U1-A, and U1-C indices as well as the anti-U1 RNP index were calculated. RESULTS: CSF anti-U1 RNP antibodies with an increased anti-U1 RNP index showed 64.3% sensitivity and 92.9% specificity for central NPSLE. The anti-U1 RNP index did not correlate with CSF IL-6 levels or the Qalb. The anti-U1-70K index was higher than the anti-U1-A and anti-U1-C indices in the CSF of anti-U1 RNP antibody-positive patients with central NPSLE. The major autoantigenic region for CSF anti-U1-70K antibodies appeared to be localized in U1-70K amino acid 141-164 residue within the RNA-binding domain. CONCLUSION: The frequency of anti-U1 RNP antibodies in the CSF and the anti-U1 RNP index are useful indicators of central NPSLE in anti-U1 RNP antibody-positive patients. The predominance of anti-U1-70K antibodies in CSF suggests intrathecal anti-U1 RNP antibody production. |
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Authors:
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Takeshi Sato; Takao Fujii; Tomoko Yokoyama; Yoshimasa Fujita; Yoshitaka Imura; Naoichiro Yukawa; Daisuke Kawabata; Takaki Nojima; Koichiro Ohmura; Takashi Usui; Tsuneyo Mimori |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Arthritis and rheumatism Volume: 62 ISSN: 1529-0131 ISO Abbreviation: Arthritis Rheum. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-12-01 Completed Date: 2011-03-09 Revised Date: 2011-10-26 |
Medline Journal Info:
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Nlm Unique ID: 0370605 Medline TA: Arthritis Rheum Country: United States |
Other Details:
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Languages: eng Pagination: 3730-40 Citation Subset: AIM; IM |
Copyright Information:
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Copyright © 2010 by the American College of Rheumatology. |
Affiliation:
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Kyoto University and National Hospital Organization Utano Hospital, Kyoto, Japan. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Antibodies, Anti-Idiotypic / cerebrospinal fluid* Biological Markers / cerebrospinal fluid Female Humans Interleukin-6 / cerebrospinal fluid Lupus Vasculitis, Central Nervous System / cerebrospinal fluid* Middle Aged Mixed Connective Tissue Disease / cerebrospinal fluid* Retrospective Studies Ribonucleoprotein, U1 Small Nuclear / cerebrospinal fluid, immunology* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Anti-Idiotypic; 0/Biological Markers; 0/Interleukin-6; 0/Ribonucleoprotein, U1 Small Nuclear; 0/SNRNP70 protein, human; 0/U1-C protein, human; 0/U1A protein |
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