Document Detail

Anti-tumour activity of fotemustine and protons in combination with bevacizumab.
MedLine Citation:
PMID:  20551638     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Metastatic melanoma is one of the most aggressive tumours and is also very resistant to current therapeutic approaches. The aim of this investigation was the in vitro study of the anti-proliferative effects of fotemustine (FM; 100 and 250 microM), bevacizumab (5 microg/ml) and proton irradiation (12 and 16 Gy) on resistant HTB140 human melanoma cells.
METHODS: Viability was estimated by sulphorhodamine B assay, while cell proliferation was analyzed by 5-bromo-2-deoxyuridine assay. Cell cycle distribution and apoptosis were examined using flow cytometry.
RESULTS: Cell viability and proliferation were reduced after all applied treatments. The level of apoptosis significantly increased after treatment with FM, protons or a combination of all agents, while the apoptotic index ranged from 1.2 to 9.2. Proton irradiation, as well as combined treatment with bevacizumab and protons or 100 microM FM, bevacizumab and protons, have reduced melanoma cell proliferation through the induction of G1 phase arrest. Single FM (250 microM) or bevacizumab treatment and their combination, as well as the joint application of these 2 agents with protons, reduced cell proliferation and provoked G2 phase accumulation.
CONCLUSION: The analyzed treatments reduced cell viability and proliferation, triggered G1 or G2 cell cycle phase accumulation and stimulated apoptotic cell death.
Lela B Korićanac; Jelena J Zakula; Ivan M Petrović; Lucia M Valastro; Giuseppe A P Cirrone; Giacomo Cuttone; Aleksandra M Ristić-Fira
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-11
Journal Detail:
Title:  Chemotherapy     Volume:  56     ISSN:  1421-9794     ISO Abbreviation:  Chemotherapy     Publication Date:  2010  
Date Detail:
Created Date:  2010-06-30     Completed Date:  2011-03-08     Revised Date:  2014-07-30    
Medline Journal Info:
Nlm Unique ID:  0144731     Medline TA:  Chemotherapy     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  214-22     Citation Subset:  IM    
Copyright Information:
Copyright 2010 S. Karger AG, Basel.
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MeSH Terms
Antibodies, Monoclonal / administration & dosage*
Antibodies, Monoclonal, Humanized
Antineoplastic Agents / administration & dosage*
Antineoplastic Combined Chemotherapy Protocols / administration & dosage
Apoptosis / drug effects,  physiology
Cell Line, Tumor
Cell Survival / drug effects,  physiology
Nitrosourea Compounds / administration & dosage*
Organophosphorus Compounds / administration & dosage*
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antibodies, Monoclonal, Humanized; 0/Antineoplastic Agents; 0/Nitrosourea Compounds; 0/Organophosphorus Compounds; 0/Protons; 2S9ZZM9Q9V/bevacizumab; 92118-27-9/fotemustine

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