Document Detail


Anti-Kp 90 IgA antibodies in the diagnosis of active tuberculosis.
MedLine Citation:
PMID:  9823997     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Accurate diagnosis of active tuberculosis (TB) has been difficult historically, yet a great demand persists for a rapid and reliable diagnostic method. Detection of Mycobacterium tuberculosis anti-Kp 90 IgA antibodies is one of the more novel techniques. STUDY OBJECTIVES: To evaluate the diagnostic value of a recently developed enzyme-linked immunosorbent assay (ELISA) test, which detects IgA antibodies against M tuberculosis Kp 90 antigen, and to compare the results with conventional diagnosis and the polymerase chain reaction (PCR) method. PARTICIPANTS: Serum, ethylenediaminetetraacetic acid (EDTA)-blood, and body fluid samples were obtained from 51 patients with active TB and 71 control subjects. The clinical diagnosis of TB was supported by a positive culture (n = 6), detection of acid-fast bacilli on smear (n = 35), or both (n = 10). MEASUREMENTS AND RESULTS: IgA antibodies were detected in sera and/or body fluid samples from 82% of patients with TB and 10% of controls. M tuberculosis DNA was detected in body fluid sample of 96% and blood sample of 49% of patients with TB by PCR. None of the blood and 5.6% of the body fluid specimens from controls were PCR-positive. CONCLUSIONS: Anti-Kp 90 IgA antibodies were detected using ELISA in 78% of serum and 69% of body fluids from patients with TB, therefore, this test is promising for the diagnosis of active TB and appears to be more reliable, particularly for body fluid samples.
Authors:
S Arikan; S Tuncer; D Us; S Unal; S Ustaçelebi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chest     Volume:  114     ISSN:  0012-3692     ISO Abbreviation:  Chest     Publication Date:  1998 Nov 
Date Detail:
Created Date:  1998-12-09     Completed Date:  1998-12-09     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0231335     Medline TA:  Chest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1253-7     Citation Subset:  AIM; IM    
Affiliation:
Hacettepe University School of Medicine, Ankara, Turkey.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Aged, 80 and over
Antibodies, Anti-Idiotypic / immunology*
Antibodies, Bacterial / analysis*
Antigens, Bacterial*
DNA, Bacterial / analysis
Enzyme-Linked Immunosorbent Assay
Female
Humans
Immunoglobulin A / analysis*
Male
Middle Aged
Mycobacterium tuberculosis / genetics,  immunology*,  isolation & purification
Polymerase Chain Reaction
Sensitivity and Specificity
Tuberculosis, Pulmonary / diagnosis*
Chemical
Reg. No./Substance:
0/Antibodies, Anti-Idiotypic; 0/Antibodies, Bacterial; 0/Antigens, Bacterial; 0/DNA, Bacterial; 0/Immunoglobulin A; 0/antigen Kp 90

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