| Anti-inflammatory and analgesic activities of SKLJI, a highly purified and injectable herbal extract of Lonicera japonica. | |
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MedLine Citation:
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PMID: 20944425 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The parenteral route has many merits over the oral route, including greater predictability, reproducibility of absorption, and rapid drug action, but injectable phytomedicines are uncommon due to protein precipitating tannin and hemolytic saponin components. In this study, in an effort to develop a safe injectable analgesic phytomedicine, we prepared a tannin and saponin-free Lonicera japonica extract, SKLJI, through fractionation and column purification, and evaluated its anti-inflammatory and analgesic activities in in vivo experimental models of inflammation and pain. The removal of tannin and saponin resulted in loganin and sweroside-enriched SKLJI and it showed reduced hemolysis and protein precipitation. In efficacy tests, SKLJI inhibited croton oil- and arachidonic acid-induced ear edema, acetic acid-induced writhing, and carrageenan-induced rat hind paw hyperalgesia. Inhibition of cylcooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), and 5-lipoxyfenase (5-LO) activities by SKLJI appeared to be the mechanism underlying anti-inflammatory and analgesic efficacy. Loganin and sweroside also showed anti-inflammatory and analgesic activities, suggesting that they might be active principles in the efficacy of SKLJI. These results suggest that SKLJI is a viable candidate for a new anti-inflammatory and analgesic phytomedicine that can be administered by the parenteral route. |
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Authors:
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Keun Ho Ryu; Hae In Rhee; Joo Hyon Kim; Hunseung Yoo; Bong Yong Lee; Key-An Um; Keunyoung Kim; Ji-Yoon Noh; Kyung-Min Lim; Jin-Ho Chung |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-07 |
Journal Detail:
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Title: Bioscience, biotechnology, and biochemistry Volume: 74 ISSN: 1347-6947 ISO Abbreviation: Biosci. Biotechnol. Biochem. Publication Date: 2010 |
Date Detail:
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Created Date: 2010-10-29 Completed Date: 2011-02-14 Revised Date: 2011-08-11 |
Medline Journal Info:
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Nlm Unique ID: 9205717 Medline TA: Biosci Biotechnol Biochem Country: Japan |
Other Details:
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Languages: eng Pagination: 2022-8 Citation Subset: IM |
Affiliation:
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Pharmacology Team, Life Science R&D Center, SK Chemical Suwon, Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Analgesics
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administration & dosage,
isolation & purification*,
pharmacology*,
therapeutic use Animals Anti-Inflammatory Agents / administration & dosage, isolation & purification*, pharmacology*, therapeutic use Arachidonate 5-Lipoxygenase / metabolism Cyclooxygenase 2 / metabolism Enzyme Inhibitors / administration & dosage, isolation & purification, pharmacology, therapeutic use Hemolysis / drug effects Inflammation / drug therapy, enzymology, immunology Injections, Intravenous Iridoid Glucosides / pharmacology, therapeutic use Iridoids / pharmacology, therapeutic use Lonicera / chemistry* Male Mice Nitric Oxide Synthase Type II / antagonists & inhibitors Plant Extracts / administration & dosage, isolation & purification*, pharmacology*, therapeutic use Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
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0/Analgesics; 0/Anti-Inflammatory Agents; 0/Enzyme Inhibitors; 0/Iridoid Glucosides; 0/Iridoids; 0/Plant Extracts; 14215-86-2/sweroside; 18524-94-2/loganin; EC 1.13.11.34/Arachidonate 5-Lipoxygenase; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 1.14.99.1/Cyclooxygenase 2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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