Document Detail


Anti-inflammatory activity of sulfur-containing compounds from garlic.
MedLine Citation:
PMID:  23057778     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We identified four anti-inflammatory sulfur-containing compounds from garlic, and their chemical structures were identified as Z- and E-ajoene and oxidized sulfonyl derivatives of ajoene. The sulfur compounds inhibited the production of nitric oxide (NO) and prostaglandin E(2) (PGE(2)) and the expression of the pro-inflammatory cytokines tumor necrosis factor-α, interleukin-1β, and interleukin-6 in lipopolysaccharide (LPS)-activated macrophages. Western blotting and reverse transcription-polymerase chain reaction analysis demonstrated that these sulfur compounds attenuated the LPS-induced expression of the inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) proteins and mRNA. Moreover, these sulfur-containing compounds suppressed the nuclear factor-κB (NF-κB) transcriptional activity and the degradation of inhibitory-κBα in LPS-activated macrophages. Furthermore, we observed that they markedly inhibited the LPS-induced phosphorylations of p38 mitogen-activated protein kinases and extracellular signal-regulated kinases (ERK) at 20 μM. These data demonstrate that the sulfur compounds from garlic, (Z, E)-ajoene and their sulfonyl analogs, can suppress the LPS-induced production of NO/PGE(2) and the expression of iNOS/COX-2 genes by inhibiting the NF-κB activation and the phosphorylations of p38 and ERK. Taken together, these data show that Z- and E-ajoene and their sulfonyl analogs from garlic might have anti-inflammatory therapeutic potential.
Authors:
Da Yeon Lee; Hua Li; Hyo Jin Lim; Hwa Jin Lee; Raok Jeon; Jae-Ha Ryu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-10-11
Journal Detail:
Title:  Journal of medicinal food     Volume:  15     ISSN:  1557-7600     ISO Abbreviation:  J Med Food     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-06     Completed Date:  2013-04-17     Revised Date:  2013-11-05    
Medline Journal Info:
Nlm Unique ID:  9812512     Medline TA:  J Med Food     Country:  United States    
Other Details:
Languages:  eng     Pagination:  992-9     Citation Subset:  IM    
Affiliation:
Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University, Seoul, Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anti-Inflammatory Agents / pharmacology*
Cell Line
Cyclooxygenase 2 / genetics,  metabolism
Extracellular Signal-Regulated MAP Kinases / genetics,  metabolism
Garlic / chemistry*
Inflammation / drug therapy
Interleukin-1beta / antagonists & inhibitors,  genetics,  metabolism
Interleukin-6 / antagonists & inhibitors,  genetics,  metabolism
Lipopolysaccharides / metabolism
Macrophages / metabolism
Mice
NF-kappa B / genetics,  metabolism
Nitric Oxide / antagonists & inhibitors,  metabolism
Nitric Oxide Synthase Type II / genetics,  metabolism
Phosphorylation
Plant Extracts / pharmacology*
Sulfur Compounds / pharmacology*
Tumor Necrosis Factor-alpha / antagonists & inhibitors,  genetics,  metabolism
p38 Mitogen-Activated Protein Kinases / genetics,  metabolism
Chemical
Reg. No./Substance:
0/Anti-Inflammatory Agents; 0/Interleukin-1beta; 0/Interleukin-6; 0/Lipopolysaccharides; 0/NF-kappa B; 0/Plant Extracts; 0/Sulfur Compounds; 0/Tumor Necrosis Factor-alpha; 10102-43-9/Nitric Oxide; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 1.14.13.39/Nos2 protein, mouse; EC 1.14.99.-/Ptgs2 protein, mouse; EC 1.14.99.1/Cyclooxygenase 2; EC 2.7.11.24/Extracellular Signal-Regulated MAP Kinases; EC 2.7.11.24/p38 Mitogen-Activated Protein Kinases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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