| Anti-IL-6 antibodies suppress myeloid cell production and the generation of CFU-c in long-term bone marrow cultures. | |
| | |
MedLine Citation:
|
PMID: 1373686 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Interleukin 6 (IL-6) is one of several hemopoietic growth factors produced by stromal cell lines derived from the adherent layer of long-term bone marrow cultures (LTBMCs). To evaluate the potential role of IL-6 in stromal cell-dependent myelopoiesis, we established LTBMCs and verified that IL-6 mRNA is transcribed by heterogeneous adherent cell layers and that IL-6 protein is present in culture supernatants. Established LTBMCs were then depleted of IL-6 by using a specific neutralizing monoclonal antibody (mAb). Cultures treated for 2-3 weeks with anti-IL-6 mAb showed decreased production of maturing myeloid cells and colony-forming progenitor cells (colony-forming units in culture, CFU-c) but not stem cells (spleen colony-forming units, CFU-s). In parallel experiments, it was also found that the addition of IL-6 to LTBMCs stimulated a marked increase in total cell production, CFU-c, and day-8 CFU-s. In sum, it appears that endogenous production of IL-6, although limiting, is essential for the normal level of myelopoiesis associated with stromal cell function in LTBMCs. |
| | |
Authors:
|
S Hudak; L Thompson-Snipes; C Rocco; J Jackson; M Pearce; D Rennick |
Related Documents
:
|
20732116 - Effect of acyclovir in vitro on lymphopoiesis in the foetal rat thymus. 18456276 - Cytokine induction by pyrogens: comparison of whole blood, mononuclear cells, and tlr-t... 11076706 - Enhanced extracellular growth of staphylococcus aureus in the presence of selected line... 9712106 - Nitric oxide downregulates interleukin 1beta (il-1beta) stimulated il-6, il-8, and pros... 2784816 - Induction of murine hemopoietic growth factors by toxic shock syndrome toxin-1. 19423096 - Estradiol modulation of hepatocyte growth factor by stromal fibroblasts in the female r... |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Experimental hematology Volume: 20 ISSN: 0301-472X ISO Abbreviation: Exp. Hematol. Publication Date: 1992 May |
Date Detail:
|
Created Date: 1992-05-26 Completed Date: 1992-05-26 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0402313 Medline TA: Exp Hematol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 412-7 Citation Subset: IM |
Affiliation:
|
Department of Immunology, DNAX Research Institute, Palo Alto, California 94304. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antibodies, Monoclonal Bone Marrow Cells* Cells, Cultured Colony-Forming Units Assay Gene Expression Granulocyte Colony-Stimulating Factor / physiology Granulocyte-Macrophage Colony-Stimulating Factor / physiology Hematopoiesis* Hematopoietic Stem Cells / cytology* Interleukin-3 / physiology Interleukin-6 / physiology* Macrophage Colony-Stimulating Factor / physiology Mice Mice, Inbred CBA RNA, Messenger / genetics |
| Chemical | |
Reg. No./Substance:
|
0/Antibodies, Monoclonal; 0/Interleukin-3; 0/Interleukin-6; 0/RNA, Messenger; 143011-72-7/Granulocyte Colony-Stimulating Factor; 81627-83-0/Macrophage Colony-Stimulating Factor; 83869-56-1/Granulocyte-Macrophage Colony-Stimulating Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Interactions among colony-stimulating factors, IL-1 beta, IL-6, and kit-ligand in the regulation of ...
Next Document: Human interleukin (IL)-9 specifically stimulates proliferation of CD34+++DR+CD33- erythroid progenit...