Document Detail

Anti-HV3 peptide antibodies as probes for conformational changes in immunoglobulins.
MedLine Citation:
PMID:  3141515     Owner:  NLM     Status:  MEDLINE    
Recent studies have shown that monoclonal anti-alprenolol antibody 37A4 could mimic the beta-adrenergic receptor with respect to binding properties and alprenolol-induced changes in intrinsic fluorescence. We prepared and characterized polyclonal antibodies against a synthetic tetradecapeptide corresponding to residues 92-105 of the 37A4 VH domain. In ELISA and immunoblotting assays, the anti-peptide antibodies bound to the intact IgG molecule as well as to the isolated heavy chain. They failed to interact with the native IgG protein but recognized with high specificity the conformationally modified Ig molecule; as a consequence, their reactivity to the entire protein was found to vary with the conditions of the assay. This study illustrates that anti-peptide antibodies might be used as probes for the detection of conformational changes occurring in the Ig hypervariable regions.
S Cazaubon; P O Couraud; J Hoebeke; C Nahmias; L Emorine; H Gras-Masse; A D Strosberg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of immunological methods     Volume:  114     ISSN:  0022-1759     ISO Abbreviation:  J. Immunol. Methods     Publication Date:  1988 Nov 
Date Detail:
Created Date:  1988-12-19     Completed Date:  1988-12-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  13-20     Citation Subset:  IM    
Laboratory of Molecular Biology of Receptors, Pasteur Institute, Paris, France.
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MeSH Terms
Antibodies, Anti-Idiotypic* / biosynthesis
Binding Sites, Antibody / drug effects
Binding, Competitive
Immunoglobulin Heavy Chains / immunology*
Immunoglobulin Variable Region / immunology*
Oligopeptides / chemical synthesis,  immunology,  pharmacology
Peptide Fragments / immunology
Protein Conformation*
Tryptophan / metabolism
Reg. No./Substance:
0/Antibodies, Anti-Idiotypic; 0/Binding Sites, Antibody; 0/Detergents; 0/Immunoglobulin Heavy Chains; 0/Immunoglobulin Variable Region; 0/Oligopeptides; 0/Peptide Fragments; 73-22-3/Tryptophan

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