Document Detail


Anti-DNA.RNA sera. Specificity tests and application in quantitative in situ hybridization.
MedLine Citation:
PMID:  6084653     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The specificity of anti-nucleic acid antisera can immunocytochemically be evaluated with test systems which apply various nucleic acids immobilized to inert matrices. When using polyrA.polyrU as a model compound for dsRNA, it is important to prevent the formation of the triple stranded form polyrA.(polyrU)2. Anti-dsRNA antibodies which, when tested with the correct test system, proved to be present in an earlier described anti-DNA.RNA serum, could be removed by adsorption. By cytofluorometric comparison of the immuno-fluorescence signals obtained with the anti-dsRNA containing serum and the absorbed serum, it could be shown that the anti-dsRNA antibodies do not contribute to the specific signals measured after in situ hybridization. Repetitive incubations with the anti-DNA.RNA serum led to a considerable increase in immunofluorescence signal.
Authors:
A K Raap; J G Marijnen; M van der Ploeg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Histochemistry     Volume:  81     ISSN:  0301-5564     ISO Abbreviation:  Histochemistry     Publication Date:  1984  
Date Detail:
Created Date:  1985-04-17     Completed Date:  1985-04-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0411300     Medline TA:  Histochemistry     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  517-20     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Nucleus / analysis
DNA / analysis*,  immunology
Enzyme-Linked Immunosorbent Assay
Immune Sera
Liver / analysis,  cytology
Mice
Nucleic Acid Hybridization*
Poly A-U
RNA / analysis*,  immunology
RNA, Double-Stranded / analysis
Chemical
Reg. No./Substance:
0/Immune Sera; 0/RNA, Double-Stranded; 24936-38-7/Poly A-U; 63231-63-0/RNA; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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