| Anti-β2GP1 antibodies have variable effects on platelet aggregation. | |
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MedLine Citation:
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PMID: 23277174 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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AIM: : To investigate the effects of affinity-purified rabbit anti-β2GP1, and anti-β2GP1 purified from patients with systemic lupus erythematosus (SLE), on adenosine diphosphate (ADP)-induced aggregation. METHODS: : Whole blood was collected and processed to obtain platelet poor plasma (PPP) from normal controls (n = 15) and SLE patients (n = 15). Using PPP, anti-β2GP1 titres were determined using an ELISA and IgG fractions isolated using a HiTrap protein G column. Anti-β2GP1 was purified from two SLE patients using purified β2GP1 coupled to a HiTrap NHS-activated HP column. RESULTS: : The effect of rabbit and human derived anti-β2GP1 (0-100 μg/mL), on ADP (2.5, 5 μM) induced platelet aggregation were investigated using light transmission aggregometry. Rabbit anti-β2GP1 significantly inhibited all parameters of 5 μM ADP-induced platelet aggregation; %Max (p = 0.028), %AUC (p = 0.014) and slope (p < 0.001). In contrast, anti-β2GP1 purified from SLE patients significantly enhanced the %Max (p = 0.031) and %AUC (p = 0.007) in a concentration dependent manner, but inhibited the slope (p < 0.05) of 5 μM ADP-induced platelet aggregation. CONCLUSION: : Our data suggest anti-β2GP1 purified from different species have variable effects on in vitro platelet aggregation. The disparity between rabbit and human anti-β2GP1 may be due to the heterogeneous nature of anti-β2GP1, varying avidity or different antibody binding specificities between species. |
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Authors:
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Natasha A Betts; Kiran D K Ahuja; Murray J Adams |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Pathology Volume: 45 ISSN: 1465-3931 ISO Abbreviation: Pathology Publication Date: 2013 Feb |
Date Detail:
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Created Date: 2013-01-01 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0175411 Medline TA: Pathology Country: England |
Other Details:
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Languages: eng Pagination: 155-61 Citation Subset: IM |
Affiliation:
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School of Human Life Sciences, University of Tasmania, Launceston, Tasmania, Australia. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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