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Anti-β2GP1 antibodies have variable effects on platelet aggregation.
MedLine Citation:
PMID:  23277174     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
AIM: : To investigate the effects of affinity-purified rabbit anti-β2GP1, and anti-β2GP1 purified from patients with systemic lupus erythematosus (SLE), on adenosine diphosphate (ADP)-induced aggregation.
METHODS: : Whole blood was collected and processed to obtain platelet poor plasma (PPP) from normal controls (n = 15) and SLE patients (n = 15). Using PPP, anti-β2GP1 titres were determined using an ELISA and IgG fractions isolated using a HiTrap protein G column. Anti-β2GP1 was purified from two SLE patients using purified β2GP1 coupled to a HiTrap NHS-activated HP column.
RESULTS: : The effect of rabbit and human derived anti-β2GP1 (0-100 μg/mL), on ADP (2.5, 5 μM) induced platelet aggregation were investigated using light transmission aggregometry. Rabbit anti-β2GP1 significantly inhibited all parameters of 5 μM ADP-induced platelet aggregation; %Max (p = 0.028), %AUC (p = 0.014) and slope (p < 0.001). In contrast, anti-β2GP1 purified from SLE patients significantly enhanced the %Max (p = 0.031) and %AUC (p = 0.007) in a concentration dependent manner, but inhibited the slope (p < 0.05) of 5 μM ADP-induced platelet aggregation.
CONCLUSION: : Our data suggest anti-β2GP1 purified from different species have variable effects on in vitro platelet aggregation. The disparity between rabbit and human anti-β2GP1 may be due to the heterogeneous nature of anti-β2GP1, varying avidity or different antibody binding specificities between species.
Authors:
Natasha A Betts; Kiran D K Ahuja; Murray J Adams
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Pathology     Volume:  45     ISSN:  1465-3931     ISO Abbreviation:  Pathology     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-01     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0175411     Medline TA:  Pathology     Country:  England    
Other Details:
Languages:  eng     Pagination:  155-61     Citation Subset:  IM    
Affiliation:
School of Human Life Sciences, University of Tasmania, Launceston, Tasmania, Australia.
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