Document Detail


Anthocyans fail to suppress transformation of aryl hydrocarbon receptor induced by dioxin.
MedLine Citation:
PMID:  15914907     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dioxins induce adverse effects through transformation of the cytosolic aryl hydrocarbon receptor (AhR). Our previous study found that flavones and flavonols at dietary levels suppress AhR transformation. In the present study, we investigated whether 20 anthocyans dissolved in trifluoroacetic acid (TFA)-MeOH suppressed AhR transformation in a cell-free system and in Hepa-1c1c7 cells. Although four compounds at 50 muM suppressed 0.1 nM 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced AhR transformation and their effects were dose-dependent in the cell-free system, they were ineffective at 0.5 muM, which is close to physiological concentration. Moreover, no anthocyan at 50 muM tested here suppressed 0.1 nM TCDD-induced AhR transformation in Hepa-1c1c7 cells. We also confirmed that protocatechuic acid and related compounds, which are possible metabolites of anthocyans, did not affect the transformation in the cell-free system. It is concluded that anthocyans are not suitable candidates for protection from dioxin toxicity.
Authors:
Rie Mukai; Itsuko Fukuda; Keizo Hosokawa; Shin Nishiumi; Atsushi Kaneko; Hitoshi Ashida
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  69     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-05-25     Completed Date:  2005-09-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  896-903     Citation Subset:  IM    
Affiliation:
Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Hyogo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anthocyanins / chemistry,  pharmacology*
Cell Line
Cell-Free System
Mice
Molecular Structure
Receptors, Aryl Hydrocarbon / drug effects*,  metabolism
Tetrachlorodibenzodioxin / antagonists & inhibitors*,  pharmacology
Chemical
Reg. No./Substance:
0/Anthocyanins; 0/Receptors, Aryl Hydrocarbon; 1746-01-6/Tetrachlorodibenzodioxin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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