Document Detail

Anthocyanin-producing dandelion callus as a chalcone synthase source in recombinant polyketide reductase assay.
MedLine Citation:
PMID:  9311152     Owner:  NLM     Status:  MEDLINE    
Purple-coloured dandelion (Taraxacum officinale) callus cultures producing anthocyanin pigments were established on a cytokinin-rich medium under the light. When the cells were placed in the dark, only grey cells proliferated. Anthocyanin productivity of these cells was partially restored in the light. The major pigment was identified as cyanidin 3-(6"-malonylglucoside). The lower stem of the original plant contained the same pigment. Chalcone synthase (CHS) activity was detected in the extracts of these purple cells, whereas no activity was observed in grey cells propagated in the dark. When the CHS-active cell-free extract was combined with the extract of Escherichia coli over expressing polyketide reductase (PKR) cDNA of licorice (Glycyrrhiza echinata), isoliquiritigenin (a 6'-deoxychalcone), in addition to naringenin (a 5-hydroxyflavanone), was detected as the reaction product from 4-coumaroyl-CoA, malonyl-CoA and NADPH. This result confirms the catalytic function of the PKR gene product.
T Akashi; N Saito; H Hirota; S Ayabe
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Phytochemistry     Volume:  46     ISSN:  0031-9422     ISO Abbreviation:  Phytochemistry     Publication Date:  1997 Sep 
Date Detail:
Created Date:  1997-10-23     Completed Date:  1997-10-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0151434     Medline TA:  Phytochemistry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  283-7     Citation Subset:  B    
Department of Applied Biological Sciences, Nihon University, Kanagawa, Japan.
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MeSH Terms
Acyltransferases / metabolism*
Anthocyanins / biosynthesis*
Bacterial Proteins / metabolism
Cells, Cultured
Escherichia coli / enzymology,  metabolism
Glycyrrhiza / enzymology
Oxidoreductases / metabolism*
Plant Extracts / metabolism,  pharmacology
Plant Proteins / metabolism*
Plants / enzymology,  metabolism*
Plants, Medicinal
Reg. No./Substance:
0/Anthocyanins; 0/Bacterial Proteins; 0/Plant Extracts; 0/Plant Proteins; EC 1.-/Oxidoreductases; EC 2.3.-/Acyltransferases; EC synthetase

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