Document Detail


Anthocyanin and flavonoid production from Perilla frutescens: pilot plant scale processing including cross-flow microfiltration and reverse osmosis.
MedLine Citation:
PMID:  16756359     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Extraction and concentration at a pilot plant scale of anthocyanins and flavonoids from Perilla frutescens var. frutescens harvested in the Guangzhou area of China were investigated. The study of extraction efficiency using mineral acids and organic acids showed that 0.01 mol/L nitric acid was the most suitable to extract flavonoids from this slightly red leaf cultivar. The red extract contained 12 mg/L (as cyanidin equivalent) anthocyanins and other flavones. The multistep process included cross-flow microfiltration (CFM) with a ceramic type membrane, reverse osmosis (RO), and rotating evaporation (RE). The filtration fluxes were high and constant for CFM (150 L/h/m2 at 0.6 b) and for RO (22 L/h/m2 at 40 b). The red extract was concentrated 9.4 times by RO and then 5.4 times by RE. It contained 422 mg/L anthocyanins, representing 77% of the total extracted anthocyanin. The proportion of flavonoids was found unchanged during processing. The concentrated extract showed a pH of 2.7, and its free acidity was found to be 46% of the acidity added for extraction, because of the buffering capacity of the extract. At the concentration level reached, a crystallized deposit occurred and was identified as tartrate.
Authors:
Linghua Meng; Yves Lozano; Isabelle Bombarda; Emile Gaydou; Bin Li
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  54     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-06-07     Completed Date:  2006-08-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4297-303     Citation Subset:  IM    
Affiliation:
Centre de Coopération Internationale en Recherche Agronomique pour le Développement, UMR Génie des Procédés et Elaboration des Bioproduits, TA 40/16, 73 Avenue Agropolis, 34398 Montpellier Cedex 5, France.
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MeSH Terms
Descriptor/Qualifier:
Anthocyanins / analysis,  isolation & purification*
Chromatography, High Pressure Liquid
Filtration
Flavones / analysis
Flavonoids / isolation & purification*
Food Handling / methods*
Osmosis
Perilla frutescens / chemistry*
Plant Extracts / chemistry
Plant Leaves / chemistry
Plant Stems / chemistry
Chemical
Reg. No./Substance:
0/Anthocyanins; 0/Flavones; 0/Flavonoids; 0/Plant Extracts

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