Document Detail


Anterior regeneration in the hemichordate Ptychodera flava.
MedLine Citation:
PMID:  18924231     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ptychodera flava is a hemichordate whose anterior structures regenerate reproducibly from posterior trunk pieces when amputated. We characterized the cellular processes of anterior regeneration with respect to programmed cell death and cell proliferation, after wound healing. We found scattered proliferating cells at day 2 of regeneration using a proliferating cell nuclear antigen antibody. On day 4, most proliferating cells were associated with the nerve tract under the epidermis, and on day 6, a small proboscis derived from proliferated cells was regenerated, and a mouth had broken though the epidermis. TUNEL (terminal deoxynucleotidyl transferase-mediated deoxyuridinetriphosphate nick end-labeling) detected elevated levels of apoptosis in the endoderm that began furthest away from the region of wound healing, then moved anteriorly over 8 days. Posterior to anterior apoptosis is likely to remove digestive endoderm for later differentiation of pharyngeal endoderm. We hypothesize that P. flava regeneration is nerve dependent and that remodeling in the gut endoderm plays an important role in regeneration.
Authors:
Amanda L Rychel; Billie J Swalla
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Developmental dynamics : an official publication of the American Association of Anatomists     Volume:  237     ISSN:  1058-8388     ISO Abbreviation:  Dev. Dyn.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-11-03     Completed Date:  2009-02-23     Revised Date:  2014-03-19    
Medline Journal Info:
Nlm Unique ID:  9201927     Medline TA:  Dev Dyn     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3222-32     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2008 Wiley-Liss, Inc.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / physiology
Cell Proliferation
Chordata, Nonvertebrate / cytology*,  physiology*
Endoderm / cytology,  physiology
Epidermis / cytology,  physiology
Peripheral Nerves / cytology,  physiology
Proliferating Cell Nuclear Antigen / metabolism
Regeneration / physiology*
Grant Support
ID/Acronym/Agency:
T32 HD007183/HD/NICHD NIH HHS; T32 HD007183-26A1/HD/NICHD NIH HHS; T32 HD007183-26A1/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Proliferating Cell Nuclear Antigen
Comments/Corrections

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