| Anterior regeneration in the hemichordate Ptychodera flava. | |
| | |
MedLine Citation:
|
PMID: 18924231 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Ptychodera flava is a hemichordate whose anterior structures regenerate reproducibly from posterior trunk pieces when amputated. We characterized the cellular processes of anterior regeneration with respect to programmed cell death and cell proliferation, after wound healing. We found scattered proliferating cells at day 2 of regeneration using a proliferating cell nuclear antigen antibody. On day 4, most proliferating cells were associated with the nerve tract under the epidermis, and on day 6, a small proboscis derived from proliferated cells was regenerated, and a mouth had broken though the epidermis. TUNEL (terminal deoxynucleotidyl transferase-mediated deoxyuridinetriphosphate nick end-labeling) detected elevated levels of apoptosis in the endoderm that began furthest away from the region of wound healing, then moved anteriorly over 8 days. Posterior to anterior apoptosis is likely to remove digestive endoderm for later differentiation of pharyngeal endoderm. We hypothesize that P. flava regeneration is nerve dependent and that remodeling in the gut endoderm plays an important role in regeneration. |
| | |
Authors:
|
Amanda L Rychel; Billie J Swalla |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
|
Title: Developmental dynamics : an official publication of the American Association of Anatomists Volume: 237 ISSN: 1058-8388 ISO Abbreviation: Dev. Dyn. Publication Date: 2008 Nov |
Date Detail:
|
Created Date: 2008-11-03 Completed Date: 2009-02-23 Revised Date: 2013-06-05 |
Medline Journal Info:
|
Nlm Unique ID: 9201927 Medline TA: Dev Dyn Country: United States |
Other Details:
|
Languages: eng Pagination: 3222-32 Citation Subset: IM |
Copyright Information:
|
Copyright (c) 2008 Wiley-Liss, Inc. |
Affiliation:
|
Biology Department, Center for Developmental Biology, and Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, Washington 98195-1800, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Apoptosis / physiology Cell Proliferation Chordata, Nonvertebrate / cytology*, physiology* Endoderm / cytology, physiology Epidermis / cytology, physiology Peripheral Nerves / cytology, physiology Proliferating Cell Nuclear Antigen / metabolism Regeneration / physiology* |
| Grant Support | |
ID/Acronym/Agency:
|
T32 HD007183-26A1/HD/NICHD NIH HHS; T32 HD007183-26A1/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Proliferating Cell Nuclear Antigen |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Comparative expression analysis of the murine palladin isoforms.
Next Document: Definitive erythropoiesis in chicken yolk sac.