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Antenatal diagnosis of fetal genotype determines if maternal hyperglycemia due to a glucokinase mutation requires treatment.
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MedLine Citation:
PMID:  22773699     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: In women with hyperglycemia due to heterozygous glucokinase (GCK) mutations, the fetal genotype determines its growth. If the fetus inherits the mutation, birth weight is normal when maternal hyperglycemia is not treated, whereas intensive treatment may adversely reduce fetal growth. However, fetal genotype is not usually known antenatally, making treatment decisions difficult.
HISTORY AND EXAMINATION: We report two women with gestational diabetes mellitus resulting from GCK mutations with hyperglycemia sufficient to merit treatment.
INVESTIGATION: In both women, DNA from chorionic villus sampling, performed after high-risk aneuploidy screening, showed the fetus had inherited the GCK mutation. Therefore, maternal hyperglycemia was not treated. Both offspring had a normal birth weight and no peripartum complications.
CONCLUSIONS: In pregnancies where the mother has hyperglycemia due to a GCK mutation, knowing the fetal GCK genotype guides the management of maternal hyperglycemia. Fetal genotyping should be performed when fetal DNA is available from invasive prenatal diagnostic testing.
Authors:
Ali J Chakera; Victoria L Carleton; Sian Ellard; Jencia Wong; Dennis K Yue; Jason Pinner; Andrew T Hattersley; Glynis P Ross
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Publication Detail:
Type:  Case Reports; Journal Article     Date:  2012-07-06
Journal Detail:
Title:  Diabetes care     Volume:  35     ISSN:  1935-5548     ISO Abbreviation:  Diabetes Care     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-08-27     Completed Date:  2013-01-11     Revised Date:  2013-09-03    
Medline Journal Info:
Nlm Unique ID:  7805975     Medline TA:  Diabetes Care     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1832-4     Citation Subset:  IM    
Affiliation:
Peninsula College of Medicine and Dentistry, University of Exeter, Exeter, UK.
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MeSH Terms
Descriptor/Qualifier:
Adult
Diabetes, Gestational / genetics
Female
Genotype
Glucokinase / genetics*
Humans
Hyperglycemia / genetics*
Mutation
Pregnancy
Prenatal Diagnosis / methods*
Chemical
Reg. No./Substance:
EC 2.7.1.2/Glucokinase
Comments/Corrections
Comment In:
Diabetes Care. 2013 Jan;36(1):e15   [PMID:  23264298 ]
Diabetes Care. 2013 Jan;36(1):e14   [PMID:  23264297 ]
Diabetes Care. 2012 Sep;35(9):1811-3   [PMID:  22923681 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-ta): Diabetes Care
Journal ID (iso-abbrev): Diabetes Care
Journal ID (hwp): diacare
Journal ID (pmc): dcare
Journal ID (publisher-id): Diabetes Care
ISSN: 0149-5992
ISSN: 1935-5548
Publisher: American Diabetes Association
Article Information
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© 2012 by the American Diabetes Association.
creative-commons:
Received Day: 23 Month: 1 Year: 2012
Accepted Day: 10 Month: 4 Year: 2012
Print publication date: Month: 9 Year: 2012
Electronic publication date: Day: 14 Month: 8 Year: 2012
Volume: 35 Issue: 9
First Page: 1832 Last Page: 1834
PubMed Id: 22773699
ID: 3425005
Publisher Id: 0151
DOI: 10.2337/dc12-0151

Antenatal Diagnosis of Fetal Genotype Determines if Maternal Hyperglycemia Due to a Glucokinase Mutation Requires Treatment
Ali J. Chakera, MBCHB12
Victoria L. Carleton, MBBS34
Sian Ellard, PHD15
Jencia Wong, PHD34
Dennis K. Yue, PHD34
Jason Pinner, MBBS6
Andrew T. Hattersley, DM12
Glynis P. Ross, MBBS3
1Peninsula College of Medicine and Dentistry, University of Exeter, Exeter, U.K.
2Department of Diabetes and Endocrinology, Royal Devon and Exeter Hospital, Exeter, U.K.
3Department of Endocrinology, Royal Prince Alfred Hospital, Sydney, Australia
4The University of Sydney, Sydney, Australia
5Department of Molecular Genetics, Royal Devon and Exeter Hospital, Exeter, U.K.
6Department of Molecular and Clinical Genetics, Royal Prince Alfred Hospital, Sydney, Australia
Correspondence: Corresponding author: Glynis P. Ross, glynis.ross@sswahs.nsw.gov.au.
[equal] A.J.C. and V.L.C. contributed equally to this study.

Heterozygous mutations in the glucokinase (GCK) gene result in mild, lifelong fasting hyperglycemia (1), typically 5.5–8.0 mmol/L. The latest International Association of Diabetes and Pregnancy Study Groups’ recommendations reinforce the advice to treat hyperglycemia in pregnancy and defines gestational diabetes mellitus (GDM) as a fasting glucose ≥5.1 mmol/L (2). Therefore, patients with GCK mutations meet the criteria for GDM and account for 1–3% of the GDM population (3). For offspring of mothers with a GCK mutation and untreated hyperglycemia, birth weight is normal if the fetus inherits the mutation but is increased by 550–700 g if the fetus is not affected (4,5). Diet has little impact on the raised maternal fasting glucose. Insulin treatment is indicated if the fetus is not affected. However, if the fetus is affected, growth is normal if the mother is not treated, and achieving maternal euglycemia may result in reduced fetal growth, so maternal treatment is not recommended (6). Usually fetal genotype is not known antenatally; therefore, regular assessment of fetal growth is recommended with acceleration of fetal abdominal circumference >75th percentile, a prudent surrogate basis for the decision of whether to treat maternal hyperglycemia (5,7).

We describe the first two cases of fetal diagnosis of a GCK mutation, which altered gestational management of maternal hyperglycemia. Both patients provided consent to publication of their case.


CASE 1
History and examination

A white, European, 33-year-old woman was diagnosed with GDM at 32 weeks’ gestation in her first pregnancy (75-g oral glucose tolerance test [OGTT] = 5.8, 9.9, and 8.0 mmol/L [fasting, 1 h, and 2 h, respectively]). Insulin treatment was given from 35 weeks’ gestation. She delivered a healthy girl, weighing 3,405 g (50th percentile), at 39 weeks’ gestation.

A heterozygous GCK mutation (c.1340G>A, p.Arg447Gln) was found in the mother on sequencing performed because of a three-generation paternal history of diabetes, persistent fasting hyperglycemia, and small glucose increment in a postpartum OGTT (7.0, 7.5, and 7.9 mmol/L) (8). During her second pregnancy, her initial self-monitored blood glucose levels were ≈6 mmol/L, fasting, and 6–8 mmol/L, 1-h postmeal. Her HbA1c was 46 mmol/mol at 12 weeks’ gestation. The hyperglycemia was managed with diet alone during the first two trimesters.

Investigation

Chorionic villus sampling (CVS) had been undertaken at 13 weeks’ gestation after high-risk first-trimester aneuploidy screening (normal result). Urgent sequencing of DNA from this sample at 18 weeks’ gestation found the fetus to be heterozygous for the GCK mutation. Thus, insulin treatment was not initiated in the second or third trimester despite fasting and postprandial hyperglycemia on self-monitoring (Fig. 1A) and the fetal abdominal circumference being in the 94th percentile. The patient’s HbA1c was 41 mmol/mol at 36 weeks’ gestation. She delivered a normal boy, weighing 3,415 g (51st percentile), at 39 weeks’ gestation. There was no neonatal hypoglycemia or peripartum complication.


CASE 2
History and examination

In her first pregnancy, a white, European, 43-year-old woman had selective fetocide at 17 weeks’ gestation for dichorionic twins discordant for trisomy 21. She delivered a healthy boy at 38 weeks, weighing 3,000 g (21st percentile). She was diagnosed with GDM in her second pregnancy. The pattern of glucose values (OGTT at 28 weeks = 5.5 and 8.3 mmol/L [fasting and 2 h]) (8), paternal, diet-treated diabetes, and BMI = 22.7 kg/m2 suggested a diagnosis of GCK hyperglycemia, which was confirmed on sequencing (heterozygous GCK mutation c.370G>A, p.Asp124Asn).

Self-monitored glucose levels were 5.2–6.7 mmol/L, fasting, and 5.8–10.2 mmol/L, 1-h postmeal (Fig. 1B). The fetal abdominal circumference was in the 88th percentile. The patient’s HbA1c was 34 mmol/mol at 33 weeks’ gestation. Treatment with insulin was considered in line with conventional guidelines of the management of GDM (2).

Investigation

CVS had been undertaken at 12 weeks’ gestation after high-risk first-trimester aneuploidy screening (normal result). Urgent sequencing of DNA from this sample confirmed the fetus had inherited the GCK mutation. Therefore, insulin treatment was not commenced.

The patient delivered a normal boy, weighing 3,590 g (55th percentile), at 38 weeks’ gestation. There was no neonatal hypoglycemia or peripartum complication.


CONCLUSIONS

In these two cases of GDM, due to a mutation in the GCK gene, antenatal genetic testing showed the fetuses had inherited the GCK mutation. As a result, neither mother received insulin treatment for established maternal hyperglycemia. The children had normal birth weights (51st and 55th percentiles) and did not have any metabolic complications.

Normal birth weight is the usual outcome of GDM resulting from a GCK mutation if the fetus inherits the mutation and maternal hyperglycemia is not treated (5). Heterozygous GCK mutations cause a regulated rise in fasting plasma glucose, typically 5.5–8.0 mmol/L (1). This raised glucose results in appropriate fetal insulin secretion because the affected fetus has the same homeostatic glucose set point as its mother (5). Insulin is not recommended for pregnancies in which the fetus has inherited the mutation because it can result in low birth weight (6). In contrast, when the fetus is unaffected, macrosomia is common, and treatment with insulin to achieve maternal euglycemia is appropriate.

The clinical dilemma of managing pregnant women with GCK mutations is due to uncertainty about the fetal genotype. Although unreliable (9), current advice is to measure fetal growth using serial ultrasound scans as a surrogate for fetal genotype, to guide diabetes and obstetric management. In pregnancies with accelerating fetal growth (abdominal circumference >75th percentile), it is assumed that the fetus has not inherited the mutation. Intensive insulin treatment should be initiated, aimed at lowering elevated blood glucose levels to normal pregnancy ranges. Large insulin doses of >1 U/kg/day (6) may be required to overcome maternal glucose homeostasis, which can be difficult to achieve without causing symptoms of hypoglycemia.

In these two cases, knowledge that the fetuses had inherited the GCK mutation allowed the maternal hyperglycemia in pregnancy to remain untreated. We recommend that if a pregnant woman with a GCK mutation has a CVS or amniocentesis for another reason, the fetal DNA should be tested for the GCK mutation. However, the 1% miscarriage rates associated with these procedures (10) means they are not routinely warranted for fetal genetic diagnosis in this situation. In the future, noninvasive prenatal diagnosis using cell-free fetal DNA or fetal cells in maternal blood (11) has the potential to determine fetal genotype and therefore influence the management of GCK-MODY (maturity-onset diabetes of the young) in pregnancy without the attendant risks associated with invasive prenatal testing.


Notes

fn1See accompanying editorial, p. Related Article(s):
1811.

Acknowledgments

No potential conflicts of interest relevant to this article were reported.

A.J.C. and V.L.C. wrote the manuscript. S.E. supervised the genetic testing and reviewed and edited the manuscript. J.W. and D.K.Y. reviewed the manuscript and advised on case 1. J.P. reviewed and edited the manuscript, advised on case 1, and arranged genetic testing of case 1. A.T.H. reviewed and edited the manuscript, advised on case 1, and was responsible for the clinical management of case 2. G.P.R. reviewed and edited the manuscript and was responsible for the clinical management of case 1. G.P.R. and A.T.H. are the guarantors of this work and, as such, had full access to all the data in the study and take responsibility for the integrity of the data and the accuracy of the data analysis.


References
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2. Metzger BE,Gabbe SG,Persson B,et al. International Association of Diabetes and Pregnancy Study Groups Consensus PanelInternational Association of Diabetes and Pregnancy Study Groups recommendations on the diagnosis and classification of hyperglycemia in pregnancy. Diabetes CareYear: 2010;33:676–68220190296
3. Ellard S,Beards F,Allen LI,et al. A high prevalence of glucokinase mutations in gestational diabetic subjects selected by clinical criteria. DiabetologiaYear: 2000;43:250–25310753050
4. Hattersley AT,Beards F,Ballantyne E,Appleton M,Harvey R,Ellard S. Mutations in the glucokinase gene of the fetus result in reduced birth weight. Nat GenetYear: 1998;19:268–2709662401
5. Spyer G,Macleod KM,Shepherd M,Ellard S,Hattersley AT. Pregnancy outcome in patients with raised blood glucose due to a heterozygous glucokinase gene mutation. Diabet MedYear: 2009;26:14–1819125755
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9. Hindmarsh PC,Geary MP,Rodeck CH,Kingdom JC,Cole TJ. Intrauterine growth and its relationship to size and shape at birth. Pediatr ResYear: 2002;52:263–26812149505
10. Alfirevic Z,Sundberg K,Brigham S. Amniocentesis and chorionic villus sampling for prenatal diagnosis. Cochrane Database Syst RevYear: 2003;3:CD00325212917956
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Figures

[Figure ID: F1]
Figure 1 

A: Median and range of capillary glucose by time of day for patient 1 showing hyperglycemia above conventional treatment targets from 29 to 31 weeks’ gestation. Horizontal lines indicate fasting and postprandial capillary glucose targets. B: Median and range of capillary glucose by time of day for patient 2 showing hyperglycemia above conventional treatment targets from 29 to 31 weeks’ gestation. Horizontal lines indicate fasting and postprandial capillary glucose targets. (A high-quality color representation of this figure is available in the online issue.)



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