Document Detail

Antcin B and its Ester Derivative from Antrodia camphorata Induce Apoptosis in Hepatocellular Carcinoma Cells Involves Enhancing Oxidative Stress Coincident with Activation of Intrinsic and Extrinsic Apoptotic Pathway.
MedLine Citation:
PMID:  21916504     Owner:  NLM     Status:  Publisher    
ABSTRACT: The triterpenoids methylantcinate B (MAB) and antcin B (AB) isolated from the medicinal mushroom Antrodia camphorata has been identified as strong cytotoxic agents against various type of cancer cells, however, the mechanisms of MAB and AB-induced cytotoxicity have not been adequately explored. This study investigated the roles of caspase cascades, ROS, DNA damage, mitochondrial disruption, and Bax and Bcl-2 proteins in MAB and AB-induced apoptosis of hepatocellular carcinoma (HCC) HepG2 cells. Here, we showed that MAB and AB induced apoptosis in HepG2 cells, as characterized by increased DNA fragmentation, cleavage of PARP, sub-G1 population, chromatin condensation, loss of mitochondrial membrane potential and release of cytochrome c. Increasing the levels of caspase-2, -3, -8, and -9 activities was involved in MAB and AB-induced apoptosis, and they could be attenuated by inhibitors of specific caspases, indicating that MAB and AB triggered caspase-dependent apoptotic pathway. Additionally, the enhanced apoptotic effect correlates with high expression of Fas, Fas ligand as well as Bax and decreased protein levels of Bcl-XL and Bcl-2, suggesting that both the extrinsic and intrinsic apoptosis pathways were involved in the apoptotic processes. Incubation of HepG2 cells with antioxidant enzymes superoxide dismutase, catalase and, antioxidants N-acetylcysteine and ascorbic acid attenuated the ROS generation and apoptosis induced by MAB and AB, which indicate that ROS plays a pivotal role in cell death. NADPH oxidase activation was observed in MAB- and AB-stimulated HepG2 cells; however, inhibition of such activation by diphenylamine significantly blocked MAB and AB-induced ROS production and increased cell viability. Taken together, our results provide the first evidence that triterpenoids MAB and AB induced a NADPH oxidase-provoked oxidative stress and extrinsic and intrinsic apoptosis as a critical mechanism of cause cell death in HCC cells.
Yun-Chih Hsieh; Yerra Koteswara Rao; Jacqueline Whang-Peng; Chi-Ying F Huang; Song-Kun Shyue; Shi-Lan Hsu; Yew-Min Tzeng
Related Documents :
11390504 - Il-12 suppression during experimental endotoxin tolerance: dendritic cell loss and macr...
12711674 - Cpg oligodeoxynucleotide 5mer-induced apoptosis in molt-4 leukaemia cells does not requ...
9710434 - Elevated interleukin-12 in progressive multiple sclerosis correlates with disease activ...
9498744 - Il-12 reverses established antigen-specific tolerance of contact sensitivity by affecti...
9174664 - The house that jak/stat built.
20231694 - Il-17rc is required for il-17a- and il-17f-dependent signaling and the pathogenesis of ...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-9-14
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  -     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-9-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Controllable DNA Condensation-Release Induced by Simple Azaheterocyclic-Based Metal Complexes.
Next Document:  Determination of PAHs and OH-PAHs in rat brain by gas chromatography tandem (triple quadrupole) mass...