| Antagonists of retinoic acid and BMP4 affect fetal mouse osteogenesis and odontoblast differentiation. | |
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MedLine Citation:
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PMID: 20303242 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Retinoic acid and bone morphogenetic protein (BMP4) are endogenous factors indispensable for the physiological development of vertebrates. The proximate aim of the present study was to investigate whether the natural compound citral (a retinoic acid synthesis inhibitor) and a monoclonal, anti-BMP4 antibody, administered to pregnant mice affect in the fetuses cranial osteogenesis and odontoblast differentiation. The present investigation was motivated by the fact that, retinoic acid inhibitors and BMP4 neutralizers may frequently contact human tissues (both intentional and unintentional, and/or unconsciously) inducing unanticipated effects. Our ultimate goal is the prevention of side effects and, future clinical implementation of the results. To this end, pregnant, white mice (balb-c Mus musculus) were intra-abdominally injected with either citral or anti-BMP4 antibody at the 9th gestational day. Newborns were processed within 5h, postnatal. Results were evaluated (a) macroscopically, (b) stereoscopically, following histochemical double staining of cartilage and osseous tissues and, (c) microscopically after (c(1)) histological staining of paraffin sections, and, (c(2)) immunohistochemical detection of apoptosis. Data indicate that in vivo administration of citral (biomimicking hypovitaminosis A) caused restriction/retardation of cranial chondrogenesis and osteogenesis. Apoptosis was not detected in teeth tissues. In vivo administration of anti-BMP4 antibody resulted in a transitory interference with the normal course of odontoblast differentiation and the production of pre-dentin, whereas, delay in the ossification also included the alveoli. Animals inspected in adulthood displayed a fairly normal phenotype. It is concluded that those two substances, under their concentrations experienced, are quite safe for the public. |
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Authors:
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Despina S Koussoulakou; Lukas H Margaritis; Stauros L Koussoulakos |
Publication Detail:
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Type: Journal Article Date: 2010-03-29 |
Journal Detail:
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Title: Pathophysiology : the official journal of the International Society for Pathophysiology / ISP Volume: 18 ISSN: 0928-4680 ISO Abbreviation: Pathophysiology Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-03-04 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9433813 Medline TA: Pathophysiology Country: Netherlands |
Other Details:
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Languages: eng Pagination: 103-9 Citation Subset: - |
Copyright Information:
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Copyright © 2010 Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
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Department of Cell Biology and Biophysics, Faculty of Biology, N. & K. University of Athens, Panepistimiopolis 15784, Greece. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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