Document Detail

Antagonistic roles of Rac and Rho in organizing the germ cell microenvironment.
MedLine Citation:
PMID:  17629483     Owner:  NLM     Status:  MEDLINE    
The capacity of stem cells to self renew and the ability of stem cell daughters to differentiate into highly specialized cells depend on external cues provided by their cellular microenvironments [1-3]. However, how microenvironments are shaped is poorly understood. In testes of Drosophila melanogaster, germ cells are enclosed by somatic support cells. This physical interrelationship depends on signaling from germ cells to the Epidermal growth factor receptor (Egfr) on somatic support cells [4]. We show that germ cells signal via the Egf class ligand Spitz (Spi) and provide evidence that the Egfr associates with and acts through the guanine nucleotide exchange factor Vav to regulate activities of Rac1. Reducing activity of the Egfr, Vav, or Rac1 from somatic support cells enhanced the germ cell enclosure defects of a conditional spi allele. Conversely, reducing activity of Rho1 from somatic support cells suppressed the germ cell enclosure defects of the conditional spi allele. We propose that a differential in Rac and Rho activities across somatic support cells guides their growth around the germ cells. Our novel findings reveal how signals from one cell type regulate cell-shape changes in another to establish a critical partnership required for proper differentiation of a stem cell lineage.
Angshuman Sarkar; Nishita Parikh; Stephen A Hearn; Margaret T Fuller; Salli I Tazuke; Cordula Schulz
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Current biology : CB     Volume:  17     ISSN:  0960-9822     ISO Abbreviation:  Curr. Biol.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-07-19     Completed Date:  2007-12-20     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9107782     Medline TA:  Curr Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1253-8     Citation Subset:  IM    
Department of Molecular Biology and Microbiology, Case Western Reserve, University School of Medicine, 10900 Euclid Avenue, Cleveland, OH 44106, USA.
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MeSH Terms
Cell Differentiation / physiology*
Drosophila Proteins / metabolism*
Drosophila melanogaster / cytology,  metabolism*
Epidermal Growth Factor / metabolism
Germ Cells / cytology,  metabolism*
Membrane Proteins / metabolism
Multiprotein Complexes / metabolism
Proto-Oncogene Proteins c-vav / metabolism
Receptor, Epidermal Growth Factor / metabolism
Signal Transduction / physiology
Testis / abnormalities,  cytology,  metabolism
rac GTP-Binding Proteins / metabolism*
rho GTP-Binding Proteins / metabolism*
Grant Support
Reg. No./Substance:
0/Drosophila Proteins; 0/Membrane Proteins; 0/Multiprotein Complexes; 0/Proto-Oncogene Proteins c-vav; 0/Rac1 protein, Drosophila; 148175-53-5/spi protein, Drosophila; 62229-50-9/Epidermal Growth Factor; EC, Epidermal Growth Factor; EC protein, Drosophila; EC GTP-Binding Proteins; EC GTP-Binding Proteins

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