Document Detail

Antagonistic effects of calcium ionophores and phorbol esters on T cell receptor mRNA levels in human thymocytes.
MedLine Citation:
PMID:  3257235     Owner:  NLM     Status:  MEDLINE    
Levels of mRNA of the T cell antigen receptors (TcR) in human thymocytes are differentially regulated in response to distinct intracellular signals. Activation of protein kinase C by the phorbol ester, tetradecanoyl phorbol acetate or other phorbol esters increases the levels of the alpha and beta T cell receptor (TcR-alpha, TcR-beta) mRNA, whereas an increase in cytosolic free Ca2+, induced by ionomycin or other Ca2+ ionophores, results in a decrease of alpha and beta TcR mRNA levels. In contrast, ionomycin increases the expression TcR-gamma mRNA whereas tetradecanoyl phorbol acetate prevents this induction. Our results suggest the existence of two opposing intracellular pathways that control expression of TcR-alpha and TcR-beta mRNA levels, on the one hand and TcR-gamma mRNA, on the other. These results provide the first evidence for antagonistic actions of protein kinase C and cytosolic-free Ca2+ on gene expression.
H Martinez-Valdez; P J Doherty; E Thompson; S H Benedict; E W Gelfand; A Cohen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  140     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  1988 Jan 
Date Detail:
Created Date:  1988-02-20     Completed Date:  1988-02-20     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  361-6     Citation Subset:  AIM; IM    
Division of Immunology/Rheumatology, Hospital for Sick Children, Toronto, Ontario, Canada.
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MeSH Terms
Calcium / metabolism
Enzyme Activation / drug effects
Ethers / pharmacology
Gene Expression Regulation / drug effects
Ionophores / pharmacology*
Nucleic Acid Hybridization
Protein Kinase C / metabolism
RNA, Messenger / antagonists & inhibitors*
Receptors, Antigen, T-Cell / analysis,  drug effects*,  genetics
T-Lymphocytes / drug effects,  metabolism*
Tetradecanoylphorbol Acetate / pharmacology*
Reg. No./Substance:
0/Ethers; 0/Ionophores; 0/RNA, Messenger; 0/Receptors, Antigen, T-Cell; 16561-29-8/Tetradecanoylphorbol Acetate; 56092-81-0/Ionomycin; 7440-70-2/Calcium; EC Kinase C

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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