Document Detail


Antagonistic activity of spent culture supernatants of lactic acid bacteria against Helicobacter pylori growth and infection in human gastric epithelial AGS cells.
MedLine Citation:
PMID:  19723205     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated the bactericidal activity and exclusion effect of 10 strains of lactic acid bacteria (LAB) isolated from different commercial food products and infant feces against Helicobacter pylori (H. pylori) in human gastric epithelial AGS cells. Antagonistic activity of spent culture supernatants (SCS) from LAB (LAB-SCS) was tested, and the content of organic acids in SCS was analyzed with high-performance liquid chromatography (HPLC). In addition, the bactericidal activities of LAB-SCS were estimated by a time-kill assay and by measuring the exclusion effect of LAB-SCS against H. pylori in AGS cells. The results showed that SCS from certain strains with higher concentrations of organic acids dramatically decreased the viability of H. pylori. We also proved that the organic acids could inhibit H. pylori adhesion and invasion of AGS cells. Furthermore, the concentration and speciation of organic acids in SCS after fermentation of LAB are important factors in the inhibition of H. pylori infection. In addition, the in vitro methods used in this study might provide for the rapid screening of potential probiotics with anti-H. pylori activity in the dairy industry.
Authors:
Wen-Hsin Lin; Chien-Ku Lin; Sen-Je Sheu; Chin-Fa Hwang; Wan-Ting Ye; Wen-Zhe Hwang; Hau-Yang Tsen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of food science     Volume:  74     ISSN:  1750-3841     ISO Abbreviation:  J. Food Sci.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-09-02     Completed Date:  2010-01-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0014052     Medline TA:  J Food Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  M225-30     Citation Subset:  IM    
Affiliation:
School of Pharmacy, China Medical Univ., Taichung City 404, Taiwan, P.R. China.
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MeSH Terms
Descriptor/Qualifier:
Acetic Acid / analysis,  metabolism,  pharmacology
Anti-Bacterial Agents / pharmacology*
Bacterial Adhesion / drug effects
Bacterial Physiological Phenomena
Cell Line
Colony Count, Microbial
Culture Media, Conditioned / chemistry,  pharmacology*
Enterococcus faecium / isolation & purification,  metabolism
Epithelial Cells / microbiology*
Feces / microbiology
Food Microbiology
Gastric Mucosa / cytology,  microbiology*
Helicobacter pylori / enzymology,  growth & development*,  isolation & purification,  pathogenicity*
Humans
Hydrogen-Ion Concentration
Infant
Lactic Acid / analysis,  metabolism,  pharmacology
Lactobacillus / isolation & purification,  metabolism
Microbial Viability
Pediococcus / isolation & purification,  metabolism
Time Factors
Urease / metabolism
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Culture Media, Conditioned; 50-21-5/Lactic Acid; 64-19-7/Acetic Acid; EC 3.5.1.5/Urease

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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