Document Detail


Anoxic carbon flux in photosynthetic microbial mats as revealed by metatranscriptomics.
MedLine Citation:
PMID:  23190731     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Photosynthetic microbial mats possess extraordinary phylogenetic and functional diversity that makes linking specific pathways with individual microbial populations a daunting task. Close metabolic and spatial relationships between Cyanobacteria and Chloroflexi have previously been observed in diverse microbial mats. Here, we report that an expressed metabolic pathway for the anoxic catabolism of photosynthate involving Cyanobacteria and Chloroflexi in microbial mats can be reconstructed through metatranscriptomic sequencing of mats collected at Elkhorn Slough, Monterey Bay, CA, USA. In this reconstruction, Microcoleus spp., the most abundant cyanobacterial group in the mats, ferment photosynthate to organic acids, CO2 and H2 through multiple pathways, and an uncultivated lineage of the Chloroflexi take up these organic acids to store carbon as polyhydroxyalkanoates. The metabolic reconstruction is consistent with metabolite measurements and single cell microbial imaging with fluorescence in situ hybridization and NanoSIMS.
Authors:
Luke C Burow; Dagmar Woebken; Ian P G Marshall; Erika A Lindquist; Brad M Bebout; Leslie Prufert-Bebout; Tori M Hoehler; Susannah G Tringe; Jennifer Pett-Ridge; Peter K Weber; Alfred M Spormann; Steven W Singer
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-11-29
Journal Detail:
Title:  The ISME journal     Volume:  7     ISSN:  1751-7370     ISO Abbreviation:  ISME J     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-19     Completed Date:  2013-09-30     Revised Date:  2014-04-01    
Medline Journal Info:
Nlm Unique ID:  101301086     Medline TA:  ISME J     Country:  England    
Other Details:
Languages:  eng     Pagination:  817-29     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Allyl Compounds
Bays
California
Carbon Cycle
Chloroflexi / classification,  genetics,  metabolism*
Cyanobacteria / classification,  genetics,  metabolism*
Estuaries*
Gene Expression Profiling
In Situ Hybridization, Fluorescence
Photosynthesis*
Phylogeny
Sulfides
Water Microbiology*
Chemical
Reg. No./Substance:
0/Allyl Compounds; 0/Sulfides; 983O8ZL28A/methylallyl trisulfide
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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