| Anoxia-resistant turtle brain maintains ascorbic acid content in vitro. | |
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MedLine Citation:
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PMID: 1784414 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The isolated turtle brain maintains intra- and extracellular concentrations of ascorbate when incubated in ascorbate-free physiological saline for as long as 24 h. After incubation for 1 h, total tissue content of ascorbate in the turtle cerebellum was the same as in unincubated controls. After 20-24 h, tissue ascorbate content remained at 65% of control levels, while extracellular ascorbate concentration, measured with carbon fiber voltammetric microelectrodes, was 56% of the initial value. For an intermediate incubation period of 6 h, reduced ascorbate content was maintained at about 80% of control levels, regardless of whether incubation was under normal conditions or in the absence of glucose or oxygen. By contrast, only 4% of the ascorbate content of guinea pig brain slices remained after a 6 h incubation. Maintenance of high levels of ascorbate by the anoxia-resistant turtle brain could be an important factor in the amelioration of oxidative injury in this tissue. Inclusion of ascorbate in media used for in vitro studies of mammalian brain tissue is recommended. |
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Authors:
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M E Rice; J Cammack |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Neuroscience letters Volume: 132 ISSN: 0304-3940 ISO Abbreviation: Neurosci. Lett. Publication Date: 1991 Nov |
Date Detail:
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Created Date: 1992-03-18 Completed Date: 1992-03-18 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7600130 Medline TA: Neurosci Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 141-5 Citation Subset: IM |
Affiliation:
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Department of Physiology and Biophysics, New York University Medical Center, NY 10016. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anoxia* / metabolism Ascorbic Acid / metabolism* Brain / metabolism*, physiology Cerebellum / metabolism Dehydroascorbic Acid / pharmacology Disease Susceptibility Extracellular Space / metabolism Female Male Osmolar Concentration Reference Values Turtles |
| Grant Support | |
ID/Acronym/Agency:
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NS-08740/NS/NINDS NIH HHS; NS-13742/NS/NINDS NIH HHS; NS-28480/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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490-83-5/Dehydroascorbic Acid; 50-81-7/Ascorbic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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