Document Detail


Anoxia-resistant turtle brain maintains ascorbic acid content in vitro.
MedLine Citation:
PMID:  1784414     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The isolated turtle brain maintains intra- and extracellular concentrations of ascorbate when incubated in ascorbate-free physiological saline for as long as 24 h. After incubation for 1 h, total tissue content of ascorbate in the turtle cerebellum was the same as in unincubated controls. After 20-24 h, tissue ascorbate content remained at 65% of control levels, while extracellular ascorbate concentration, measured with carbon fiber voltammetric microelectrodes, was 56% of the initial value. For an intermediate incubation period of 6 h, reduced ascorbate content was maintained at about 80% of control levels, regardless of whether incubation was under normal conditions or in the absence of glucose or oxygen. By contrast, only 4% of the ascorbate content of guinea pig brain slices remained after a 6 h incubation. Maintenance of high levels of ascorbate by the anoxia-resistant turtle brain could be an important factor in the amelioration of oxidative injury in this tissue. Inclusion of ascorbate in media used for in vitro studies of mammalian brain tissue is recommended.
Authors:
M E Rice; J Cammack
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neuroscience letters     Volume:  132     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  1991 Nov 
Date Detail:
Created Date:  1992-03-18     Completed Date:  1992-03-18     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  141-5     Citation Subset:  IM    
Affiliation:
Department of Physiology and Biophysics, New York University Medical Center, NY 10016.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anoxia* / metabolism
Ascorbic Acid / metabolism*
Brain / metabolism*,  physiology
Cerebellum / metabolism
Dehydroascorbic Acid / pharmacology
Disease Susceptibility
Extracellular Space / metabolism
Female
Male
Osmolar Concentration
Reference Values
Turtles
Grant Support
ID/Acronym/Agency:
NS-08740/NS/NINDS NIH HHS; NS-13742/NS/NINDS NIH HHS; NS-28480/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
490-83-5/Dehydroascorbic Acid; 50-81-7/Ascorbic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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