| Anoxia or oxygen and glucose deprivation in SH-SY5Y cells: a step closer to the unraveling of neuroglobin and cytoglobin functions. | |
| | |
MedLine Citation:
|
PMID: 17532579 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Several studies support the hypothesis that neuroglobin and cytoglobin play a protective role against cell death when cellular oxygen supply is critical. Although the underlying molecular mechanisms are unknown, previous reports suggest that this protection can be realised by the fact that they act as ROS scavengers. In this study, expression of neuroglobin and cytoglobin was evaluated in a human neuroblastoma cell line (SH-SY5Y) under conditions of anoxia or oxygen and glucose deprivation (OGD). The cells could survive prolonged anoxia without significant loss of viability. They became anoxia sensitive when deprived of glucose. OGD induced significant cell death after 16 h resulting in 54% dead cells after 32 h. Necrosis was the main process involved in OGD-induced cell death. After reoxygenation, apoptotic neurons became more abundant. Real-time quantitative PCR and Western blotting revealed that neuroglobin and cytoglobin were upregulated, the former under OGD and the latter under anoxic conditions. Under OGD, cell survival was significantly reduced after inhibiting cytoglobin expression by transfection with antisense ODN. Moreover, cell survival was significantly enhanced by neuroglobin or cytoglobin overexpression. When neuroglobin or cytoglobin protein expression increased or decreased, the H(2)O(2) level was found to be lower or higher, respectively. We conclude that neuroglobin or cytoglobin act as ROS scavengers under ischemic conditions. |
| | |
Authors:
|
Elke Fordel; Liesbet Thijs; Wim Martinet; Dorien Schrijvers; Luc Moens; Sylvia Dewilde |
Related Documents
:
|
7320079 - Oxygen gradients cause pattern orientation in dictyostelium cell clumps. 9337489 - Membrane-linked systems preventing superoxide formation. 7107399 - Evidence suggesting that the mechanism for aerobic and hypoxic cytotoxicity of nitrohet... 19902029 - Cardiomyocyte h9c2 cells exhibit differential sensitivity to intracellular reactive oxy... 11797039 - Assay of atp in intact cells of the facultative phototroph rhodobacter capsulatus expre... 7796889 - Cell cycle-related changes in f-actin distribution are correlated with glycolytic activ... 17142819 - Modelling radiation-induced bystander effect and cellular communication. 21573569 - A comparative-analysis of the activity of retinoic acid and cyclic-amp in the induction... 204459 - Purine biosynthesis in mutant mammalian cells. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-04-25 |
Journal Detail:
|
Title: Gene Volume: 398 ISSN: 0378-1119 ISO Abbreviation: Gene Publication Date: 2007 Aug |
Date Detail:
|
Created Date: 2007-07-30 Completed Date: 2007-09-26 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7706761 Medline TA: Gene Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 114-22 Citation Subset: IM |
Affiliation:
|
Department of Biomedical Sciences, University of Antwerp, Universiteitsplein 1, T.1, 2610 Wilrijk, Belgium. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Apoptosis
/
drug effects*,
genetics,
physiology Blotting, Western Cell Hypoxia Cell Line, Tumor Cell Survival / drug effects, genetics, physiology Flow Cytometry Gene Expression Globins / genetics, metabolism, physiology* Glucose / metabolism, pharmacology* Humans Hydrogen Peroxide / metabolism Nerve Tissue Proteins / genetics, metabolism, physiology* Neuroblastoma / metabolism, pathology Oligonucleotides, Antisense / genetics Oxygen / metabolism, pharmacology RNA, Messenger / genetics, metabolism Reactive Oxygen Species / metabolism Time Factors Transfection |
| Chemical | |
Reg. No./Substance:
|
0/Nerve Tissue Proteins; 0/Oligonucleotides, Antisense; 0/RNA, Messenger; 0/Reactive Oxygen Species; 0/neuroglobin; 50-99-7/Glucose; 7722-84-1/Hydrogen Peroxide; 7782-44-7/Oxygen; 9004-22-2/Globins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Detection of ica genes and slime production in a collection of Staphylococcus epidermidis strains fr...
Next Document: Thyroid hormone responsive genes in the murine hepatocyte cell line AML 12.