| Anorexia in rats caused by a valine-deficient diet is not ameliorated by systemic ghrelin treatment. | |
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MedLine Citation:
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PMID: 20006681 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rodents exhibit aversive behavior toward a diet that lacks at least one of the essential amino acids. We sought to determine whether the particular form of anorexia caused by such diets could be ameliorated by the administration of orexigenic peptides while simultaneously analyzing the neural mechanisms underlying anorexia. Rats were fed a valine-deficient diet, which induced severe anorexia (reducing food consumption by 80%). The severe anorexia was associated with a significant decrease in the cerebrospinal fluid valine concentration and hyper-ghrelinemia. Between 6 and 12 days after initiation of the valine-deficient diet, we injected rats twice daily with valine and/or an orexigenic peptide (ghrelin, neuropeptide Y, or agouti-related protein) either i.p. or i.c.v.. We then measured dietary intake. An i.c.v. valine injection allowed earlier food intake compared with an i.p valine injection and increased the density of c-Fos-positive ependymal cells lining the third ventricle. Whereas an i.c.v. injection of ghrelin or neuropeptide Y increased consumption of the valine-deficient diet, i.p injection of ghrelin or i.c.v. injection of agouti-related protein did not. Following i.c.v. administration of either valine or ghrelin, we did not observe complete recovery of consumption of the valine-deficient diet. This may be due to the ineffectiveness of peripheral ghrelin and central agouti-related protein and/or to conditioned aversion to the valine-deficient diet. Since ghrelin is known to be involved in food anticipatory activities, whether the hyper-ghrelinemia observed in valine-deficient rats play role in foraging behavior other than food intake is the future study to be investigated. |
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Authors:
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S Goto; K Nagao; M Bannai; M Takahashi; K Nakahara; K Kangawa; N Murakami |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-12-17 |
Journal Detail:
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Title: Neuroscience Volume: 166 ISSN: 1873-7544 ISO Abbreviation: Neuroscience Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-02-01 Completed Date: 2010-05-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7605074 Medline TA: Neuroscience Country: United States |
Other Details:
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Languages: eng Pagination: 333-40 Citation Subset: IM |
Copyright Information:
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Copyright 2010 IBRO. Published by Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Veterinary Physiology, Miyazaki University, Miyazaki, Miyazaki 889-2192, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Agouti-Related Protein
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metabolism,
pharmacology Animals Anorexia / drug therapy, metabolism*, physiopathology Appetite / drug effects, physiology* Appetite Regulation / drug effects, physiology* Dietary Proteins / metabolism Disease Models, Animal Ependyma / cytology, metabolism Exploratory Behavior / drug effects, physiology Feeding Behavior / drug effects, physiology Food, Formulated Ghrelin / metabolism*, pharmacology Hypothalamus / cytology, drug effects, metabolism Male Neuropeptide Y / metabolism, pharmacology Rats Rats, Wistar Third Ventricle / cytology, metabolism Valine / cerebrospinal fluid, deficiency*, pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Agouti-Related Protein; 0/Dietary Proteins; 0/Ghrelin; 0/Neuropeptide Y; 7004-03-7/Valine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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